Rapid Detection and Quantitation of Fungal Spores from Dust Samples Using Real-Time PCR

Details

• Personal Author:
  Chen B ; Kashon M ; Keswani J
• Description:
  Recent advances in real-time PCR have permitted accurate, rapid and quantitative identification of microorganisms in pure cultures regardless of viability or culturability. In this study, a simple sample processing method was investigated for rapid identification and quantitation of fungal spores from dust samples using real-time PCR. The proposed method was evaluated for susceptibility to interference from environmental dust samples. The extent of inhibition was calculated using real-time PCR reactions containing Aspergillus fumigatus spores specific primers and probe and various amounts of dust. No interference (p < 0.05) was detected from 0.2 mg of four real-world dust samples. However, dusts weighing > 0.2 mg compromised the assay. The overall results suggest the potential usefulness of our method for monitoring indoor microbial aerosols containing dusts weighing < / = 0.2 mg using real-time PCR. [Description provided by NIOSH]
• Subjects:
  Aerosols Dust Fungi Fungicide, Industrial Microbiology Occupational Health Safety
• Keywords:
  Aerosol-particles Aerosol-sampling Dust-particles Dust-sampling Dusts Fungicides Microorganisms
• ISBN:
  9780970991515
• Publisher:
  Fungal Research Group Foundation, Inc.
• Document Type:
  Text
• Genre:
  Proceedings
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  366-374
• NIOSHTIC Number:
  nn:20030922
• Citation:
  Bioaerosols, fungi, bacteria, mycotoxins and human health: patho-physiology, clinical effects, exposure assessment, prevention and control in indoor environments and work. Johanning E, ed., Albany, NY: Fungal Research Group Foundation, Inc., 2005 Mar; :366-374
• Email:
  jkeswani@cdc.gov
• Editor(s):
  Johanning E
• Federal Fiscal Year:
  2005
• Peer Reviewed:
  False
• Source Full Name:
  Bioaerosols, fungi, bacteria, mycotoxins and human health: patho-physiology, clinical effects, exposure assessment, prevention and control in indoor environments and work
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:766e0c8d51683233392e99c417e5ed4185369d34223cf3acf23716d5c80f783094b603b74d6c26634acd4343d4abf6e8f7c3bfac9a9c6ec99c3fdf3b746f8cb3
• Download URL:
  https://stacks.cdc.gov/view/cdc/191358/cdc_191358_DS1.pdf
• File Type:
  [PDF - 1.33 MB ]