Aerosolization of Microorganisms from Metalworking Fluids

Details

• Personal Author:
  Adhikari A ; Grinshpun SA ; Martuzevicius D ; Reponen T ; Wang HX ; Willeke K
• Description:
  Workers exposed to metal working fluid (MWF) aerosols may be at risk of developing a variety of respiratory and skin diseases. Microorganisms present as contaminants in MWFs are suspected to be one of the causative agents for respiratory symptoms and diseases in workers. Until now, most of the research has been focused on microorganisms in MWF reservoirs. Limited information is available on the composition and concentration of airborne microorganisms at metalworking sites. The aim of this study is to characterize the aerosolization of different microorganisms from MWFs. First phase of this study included aerosolization of Pseudomonas fluorescence cells, Bacillus subtilis endospores, and Penicillium melinii spores by a Collison nebulizer from three different suspensions: sterilized water, 5%semi-synthetic MWF and 5% soluble oil MWF. The resulting bioaerosols were dried, charge-neutralized and passed into the measurement chamber. The airborne microorganisms in the measurement chamber were sampled by identical Button Personal Inhalable Aerosol Samplers at an airflow rate of 4 liters/min. The concentrations in the nebulizer suspensions and on the Button Sampler filters were analyzed by microscopic enumeration. The microorganism concentration in the suspension was adjusted to 10^7 particles/ml. The concentration of airborne microorganisms ranged from 3xl0/\6 to 2x10^7 particles/m3. The aerosolization ratio the concentration in the air, #/m3 divided by the concentration in the nebulizer suspension, #/mL) varied from 0.4 to 0.7. It was found that the aerosolization of microorganisms does not depend on the type of microorganism or the type of MWF. Future experiments will be performed using a simulator for MWF aerosol generation to investigate the effect of operational parameters (rotation speed, fluid application rate, ventilation rate) on the aerosolization efficiency. [Description provided by NIOSH]
• Subjects:
  Aerosols Metals Metal Workers Occupational Exposure Occupational Health Safety
• Keywords:
  Aerosol Particles Metalworking Fluids Metalworking Industry Occupational Exposure
• Publisher:
  American Conference of Governmental Industrial Hygienists
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  12
• NIOSHTIC Number:
  nn:20030708
• Citation:
  Proceedings of the Health Effects of Mineral Oil Mist and Metalworking Fluids Symposium, October 2-4, 2002, Cincinnati, Ohio. Cincinnati, OH: American Conference of Governmental Industrial Hygienists, 2002 Oct; :12
• Federal Fiscal Year:
  2003
• NORA Priority Area:
  Work Environment and Workforce: Mixed Exposures
• Performing Organization:
  University of Cincinnati, Cincinnati, Ohio
• Peer Reviewed:
  False
• Start Date:
  20010930
• Source Full Name:
  Proceedings of the Health Effects of Mineral Oil Mist and Metalworking Fluids Symposium, October 2-4, 2002, Cincinnati, Ohio
• End Date:
  20040929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:8077a0de1f9e87d505fe581704eae97b8decfd79c8358167451fe3e71fb2ecb34a2b8b114626b0e1de32b886561dd01b30c79c5a8fbbd5b204d08a37e578a980
• Download URL:
  https://stacks.cdc.gov/view/cdc/191233/cdc_191233_DS1.pdf
• File Type:
  [PDF - 247.78 KB ]