Enhanced Production of Prostaglandin E2 (PGE2) by Alveolar Macrophage (AM) Harvested from Silica-Exposed Rats: Possible Regulatory Role of Nitric Oxide (NO)

Details

• Personal Author:
  Barger MW ; Castranova, Vincent ; Ma JKH ; Ma JYC ; Yang HM
• Description:
  This study investigated whether NO plays a significant role in regulating the production of PGE2, or inflammatory cytokines by silica-exposed AM. Rats were treated by intratracheal instillation of saline (control) or silica (20 mg/rat) and AM harvested by bronchoalveolar lavage at I, 3, 7, 14, and 28 days post-exposure. AM were cultured for 24 br and ex vivo production of PGE2 leukotriene B4 (LTB4), TNF-A, IL-1 , and NO were monitored. In vivo exposure to silica resulted in an increase in PGE, production which peaked (185 fold) at 3 days postexposure, while LTB4 release was decreased. Silica exposure incn:ased NO production by 9 fold at 3 days postexposure, while causing a moderate increase in TNF -4 production and a significant elevation of IL-1 secretion which was sustained through 28 days post-exposure. Tetrandrine, an antifibrotic agent, inhibited IL-1 and TNF-4 production but did not affect NO or PGE2 release. Indomethacin blocked PGE, production completely and bad a moderate effect on NO production. Furthermore, inhibition of NO production with N-nitro-Larginine methyl ester enhanced PGE, production without affecting TNF -4 or IL-1 release. These results suggest that silica-induced stimulation of PGE, production is controlled via a different pathway than cytokine production and that NO may play a role in limiting PGE2 production in silica-exposed rats. [Description provided by NIOSH]
• Subjects:
  Aluminum Silicates Animals Environmental Monitoring Laboratories Lung Occupational Health Respiratory System Safety Silicon Dioxide
• Keywords:
  Alveolar-cells Animal-studies Dose-response Exposure-assessment Exposure-levels Inhalation-studies Laboratory-animals Silica-dusts Silicates Silicosis
• ISSN:
  0892-6638
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  District of Columbia ; OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  13
• Issue:
  4
• NIOSHTIC Number:
  nn:20030689
• Citation:
  FASEB J 1999 Mar; 13(4)(Pt 1):A505
• CAS Registry Number:
  Nitric oxide (CAS RN 10102-43-9)
• Federal Fiscal Year:
  1999
• Peer Reviewed:
  False
• Part Number:
  1
• Source Full Name:
  The FASEB Journal
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:178ec751a5e0558a7da47d983861a7654ee4b2f744a4cc26f7bd485d8ee3d93d07bddfe71fc90586fca6698ef4e708f6eeea955515710b966a089b8cf1bac653
• Download URL:
  https://stacks.cdc.gov/view/cdc/191217/cdc_191217_DS1.pdf
• File Type:
  [PDF - 401.39 KB ]