Effects of Tetrandrine on Macrophage Production of Cytokines Resulting from Silica or Bleomycin Exposure

Details

• Personal Author:
  Barger MW ; Castranova, Vincent ; Ma JKH ; Ma JYC
• Description:
  Alveolar macrophage (AM) derived cytokines including interleukin-1 (IL1) and tumor necrosis factor-a (TNF-a) play an important role in the development of pulmonary fibrosis. Tetrandrine (TT) has been shown previously to reduce silica-induced pulmonary cell damage and collagen formation. The present objective was to investigate the effects of TT on AM production of IL-1 and TNF-a in response to silica or bleomycin (BLM). AM exposed to silica in vitro exhibited increased release of IL-1 and TNF-a. This increase was effectively reduced in the presence of TT. For in vivo studies, rats were intratracheally instilled with saline as control, 20 mg silica, or 1 mg/kg BLM. TT was administered orally (18 mg/kg) twice before exposure and 3 times/ week after exposure for desired time periods. These studies showed that in vivo exposure to silica or BLM yielded AM which produced elevated levels of IL-1 and TNF-a in culture. This production was inhibited by oral treatment with TT. These results indicate that TT treatment inhibits the silica- or BLM- stimulated production of IL-1 and TNF-a. These effects may explain in part the ability of TT to decrease silica induced fibrosis. [Description provided by NIOSH]
• Subjects:
  Aluminum Silicates Animals Environmental Monitoring Fibrosis Laboratories Occupational Health Respiratory Tract Diseases Safety Silicon Dioxide
• Keywords:
  Animal-studies Cell-damage Collagen-fibrils Exposure-assessment Exposure-levels In-vitro-studies In-vitro-study In-vivo-studies In-vivo-study Laboratory-animals Pulmonary-system-disorders Silica-dusts Silicates

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  30
• Issue:
  1
• NIOSHTIC Number:
  nn:20030647
• Citation:
  Toxicologist 1996 Mar; 30(1)(Pt 2):139
• Federal Fiscal Year:
  1996
• Peer Reviewed:
  False
• Part Number:
  2
• Source Full Name:
  The Toxicologist. Society of Toxicology 35th Annual Meeting, March 10-14,1996, Anaheim, California
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:65e41ebb570b11fe5c37b132c523e8ce94eb4d4d1b502987e5585d39388ea319cfd28cc173ce26c875e8d8a47d7b43f5b33d41e5ceedbdcca72bce4f0bac5550
• Download URL:
  https://stacks.cdc.gov/view/cdc/191188/cdc_191188_DS1.pdf
• File Type:
  [PDF - 132.81 KB ]