Developmental Toxicity of L-Phenylalanine in Intact Drosophila

Details

• Personal Author:
  Lynch DW
• Description:
  In order to further characterize the Drosophila bioassay for screening developmental toxicants, l-phenylalanine (LPA; CAS 63-91-2), a documented human and animal teratogen, was evaluated using our published protocol (Teratogenesis, Carcinogenesis, and Mutagenesis 11:147-173, 1-91). Drosophila were exposed throughout development (egg through third instar larva) in culture vials to medium containing 0-140 mg/vial LPA. Each vial contained 1g of powered medium and 5ml of distilled deionized water or a solution of test chemical in water. A mated, untreated, Oregon-R wild-type female (MidAmerican Drosophila Stock Center) was added to each culture vial and allowed to oviposit for 20 hours, then removed. Emerging offspring were collected over 10 days, and examined microscopically (25 X ) for bent humeral bristles and wing blade notches, morpohological defects shown to occur with an increased incidence in files exposed to developmental toxicants. Mortality ranged from 33% in the controls to 7S% in the 140 mg/vial LPA group. The incidence of bent bristles was statistically increased (p<0.05) compared to concurrent controls (chi-square) at all LPA concentrations, and ranged from 1.0% (2/209) in the controls to 8.4% (10/119) in the 123 mg/vial group, the estimated LC. No wing blade notches were observed. These results provide additional support for increased utilization of this test as a prescreen for developmental toxicants. [Description provided by NIOSH]
• Subjects:
  Animals Environmental Monitoring Hazardous Substances Laboratories Morbidity Mortality Occupational Health Safety Teratogenesis
• Keywords:
  Animal-studies Exposure-assessment Exposure-levels Exposure-limits Laboratory-animals Morbidity-rates Mortality-data Mortality-rates Teratogens Toxic-materials Toxins

  More +
• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DBBS - Division of Biomedical and Behavioral Sciences
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  36
• Issue:
  1
• NIOSHTIC Number:
  nn:20030611
• Citation:
  Toxicologist 1997 Mar; 36(1)(Pt 2):261
• CAS Registry Number:
  L-Phenylalanine (CAS RN 63-91-2)
• Federal Fiscal Year:
  1997
• Peer Reviewed:
  False
• Part Number:
  2
• Source Full Name:
  The Toxicologist. Society of Toxicology 36th Annual Meeting, March 9-13, 1997, Cincinnati, Ohio
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:f0a2201d09ff79fcd7bef590de1cc21d2fd8e23f07ae508053031aa5322dc7b49f7a4cf3809c133011dd0927250cf85c8a0bc334cdf2e0b9bfefcee85c75ee73
• Download URL:
  https://stacks.cdc.gov/view/cdc/191160/cdc_191160_DS1.pdf
• File Type:
  [PDF - 151.13 KB ]