Nitric Oxide-Induced Prostaglandin Release by Rat Alveolar Macrophages

Details

• Personal Author:
  Blake TL ; Castranova, Vincent
• Description:
  There is a growing body of evidence demonstrating links between the mtnc oxide synthase and cyclooxygenase pathways. We hypothesized that nitric oxide (NO) might increase the release of prostaglandins (PG) by alveolar macrophages (AM). AM were obtained from male Sprague-Dawley rats by bronchoalveolar lavage and were cultured for 6 hours:!: spermine-NO, a NO-liberating compound. Some cultures also contained superoxide dismutase (SOD). At the end of the culture period, cell supernatants were collected and assayed for NO products (Greiss method) and PGE2 (enzyme-linked immunoassay). The spermine-NO complex increased NO products in the culture medium by 19-fold (22.5 vs. 424 J-lM). Such elevated NO levels increased PGE2 release from AM 7-fold (73.3 vs. 545 pg/ml). SOD increased NO products in some cultures and had no effect in other cultures; in all cultures SOD increased PGE2 release an average of 4-fold (73.3 vs 314 pg/ rol). The combination of spermine-NO and SOD elevated PGE2 (770 pg/ml). This effect of SOD on NO-induced PGE2 release appeared to be additive, not synergistic. These results demonstrate that NO increases the release of PGE2 from AM. Induction of NO production has been associated with in vivo exposure to various particulates. Since PG have been shown to decrease fibroblast proliferation, NO-enhanced PGE2 release could represent a controlling or counterbalancing mechanism which may limit particle-induced fibrogenesis. [Description provided by NIOSH]
• Subjects:
  Animals Environmental Monitoring Hazardous Substances Laboratories Lung Lung Diseases Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Alveolar-cells Animal-studies Exposure-assessment Exposure-levels Exposure-limits Laboratory-animals Lung-cells Lung-disorders Lung-tissue Pulmonary-system-disorders Respiratory-system-disorders Toxic-dose Toxic-materials Toxins

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  Ohio ; OSHA Region 3 ; OSHA Region 5 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  77
• Volume:
  36
• Issue:
  1
• NIOSHTIC Number:
  nn:20030583
• Citation:
  Toxicologist 1997 Mar; 36(1)(Pt 2):77
• CAS Registry Number:
  Nitric oxide (CAS RN 10102-43-9)
• Federal Fiscal Year:
  1997
• Peer Reviewed:
  False
• Part Number:
  2
• Source Full Name:
  The Toxicologist. Society of Toxicology 36th Annual Meeting, March 9-13, 1997, Cincinnati, Ohio
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:b627ba58233b0d8a53dd2dfd0b502b173d400648ad88b4be7da8cedaed88976595a527d26e15ebbc1355a027ab66270dcd235e4fe3be1918a25f46a397c76efc
• Download URL:
  https://stacks.cdc.gov/view/cdc/191140/cdc_191140_DS1.pdf
• File Type:
  [PDF - 136.59 KB ]