Migration-Activated Caveolin Rear Polarization Is Controlled by a Specific Domain at the N-Terminus

Details

• Personal Author:
  Beardsley AR ; Castranova, Vincent ; Flynn DC ; Liu J ; Sun XH
• Description:
  Angiogenic growth factor-induced endothelial cell migration is a key step towards tumor angiogenesis. When cells are migrating, caveolin-1, the principle protein component of caveolae, is excluded from the leading edge and polarized to the cell rear. The migration-stimulated caveolin rear translocation appears to play an important role in endothelial cell polarization and directional movement. In the present study, we generated a series of domain deletion mutants of caveolin-1 as GFP-fusion proteins and investigated domain or sequence that was required for caveolin-1 polarization in migrating cells. Our results show that wild-type caveolin-1, Cav1-178-GFP, was polarized to the cell rear in migrating caveolin-1 deficient mouse embryonic fibroblasts (MEFs). In contrast, deletion of aa 1-60 prevented the molecule from polarization and, in turn, some of the mutant were located at the leading edge, suggesting that aa 1-60 were essential for caveolin-1 polarization. Interestingly, deletion of aa 1-60 did not prevent caveolin-1 polarization in HUVECs as well as wild-type MEFs, suggesting that Cav61-178-GFP mutant might be interacting with endogenous caveolin-1 during polarization. Consistent with this, Cav61-178-GFP and Cav61-178-Flag were co-immunoprecipitated with endogenous caveolin-1. Furthermore, Cav61-178 was incorporated into light membrane domains and mediated caveolae formation. Our results indicate that aa 1-60 in caveolin-1 were essential for its polarization in migrating cells. [Description provided by NIOSH]
• Subjects:
  Animals Growth Laboratories Occupational Health Safety
• Keywords:
  Animal-studies Cell-growth Cell-migration Growth-factors Laboratory-animals
• ISSN:
  0197-016X
• Publisher:
  American Association for Cancer Research
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  47
• NIOSHTIC Number:
  nn:20029955
• Citation:
  97th AACR Annual Meeting, April 1-5, 2006, Washington D.C. Philadelphia, PA: American Association for Cancer Research, 2006 Apr; 47:LB-201
• Federal Fiscal Year:
  2006
• Peer Reviewed:
  False
• Source Full Name:
  97th American Association for Cancer Research Annual Meeting, April 1-5, 2006, Washington D.C.
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:f1ee18487981832adebc4eeb89dafbeb9de070d00d57f20274900301c9d3385d400e619c305d785c8944b5fdfb976a2e4db46f8d30b45842f7844cfebc83022f
• Download URL:
  https://stacks.cdc.gov/view/cdc/190826/cdc_190826_DS1.pdf
• File Type:
  [PDF - 198.67 KB ]