Transforming Growth Factor-Beta in Skeletal Muscle Strain Injury

Details

• Personal Author:
  Alway SE ; Miller, G. Roger ; Smith CA ; Stauber WT
• Description:
  The presence of transforming growth factor-beta (TGF-beta) in skeletal muscle has been reported in various muscle disease. In this study, we assess the presence of transforming growth factor-beta in strain-induced muscle injury. Muscle strains were produced by manually stretching (50 repetitions) an activated plantar flexor group through its normal range of motion. Muscle biopsy samples were evaluated 24- and 48- hours after strain injury for the presence of transforming growth factor-beta using immunohistochemical techniques and Western immunoblot analysis. Transcript levels for collagen I, collagen III, and transforming growth factor-beta were assessed using reverse transcriptase polymerase chain reaction (RT-PCR). Immunohistochemical analysis revealed the presence of TGF-beta2 in areas of myofiber injury, whereas TGF-beta1 was in areas of cell necrosis and phagocytosis. RT-PCR indicated increases in collagen I, collagen III, and TGF-beta1 transcripts 24 hours post-strain injury. Increases in collagen I and collagen III transcripts were more pronounced 48 hours after strain injury. Western immunoblot analysis showed no apparent change in TGF-beta2 levels with injury, but an increase in TGF-beta1 levels which was most pronounced 48 hours post-strain injury. These results indicate that TGF-beta1 levels increase after acute strain injury following increases in TGF-beta1 mRNA correlating with increases in collagen I and collagen III transcripts, whereas TGF-beta2 may be a common resident in skeletal muscle awaiting activation. [Description provided by NIOSH]
• Subjects:
  Communications Media Mining Noise Noise, Occupational Occupational Health Safety
• Keywords:
  Diseases Injuries Injury Prevention Muscles Muscle Tissue Musculoskeletal System Musculoskeletal System Disorders Skeletal System
• ISSN:
  0195-9131
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  33
• Issue:
  5
• NIOSHTIC Number:
  nn:20029544
• Citation:
  Med Sci Sports Exerc 2001 May; 33(5)(Suppl 1):S230
• Federal Fiscal Year:
  2001
• NORA Priority Area:
  Disease and Injury: Musculoskeletal Disorders of the Upper Extremities
• Performing Organization:
  West Virginia University, Morgantown, West Virginia
• Peer Reviewed:
  False
• Start Date:
  19920101
• Source Full Name:
  Medicine and Science in Sports and Exercise
• Supplement:
  1
• End Date:
  20050531
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7fcfe7e55ce877a36356059953dd6ca9a5e373d5a35d0285248ee615065f980ede085c1ff448db28c93a7b7227b1a498085a2418199245388f7a92ac355635fb
• Download URL:
  https://stacks.cdc.gov/view/cdc/190499/cdc_190499_DS1.pdf
• File Type:
  [PDF - 37.07 KB ]