Characterization of Nigerlysin©, Hemolysin Produced by Aspergillus niger, and Effect of Mouse Neuronal Cells In Vitro

Details

• Personal Author:
  Beezhold, Donald H. ; De Jesus V ; Donohue M ; Hettick JM ; Hud N ; Schmechel D ; Vesper S ; Wei W ; Wu J ; Zawia NH
• Description:
  Aspergillus niger produced a proteinaceous hemolysin, nigerlysin (c) when incubated on sheep's blood agar (SBA) at both 23 and 37 degrees C. Nigerlysin was purified from tryptic soy broth (TSB) culture filtrate and found to have a molecular weight of approximately 72kDa, with an isoelectric point of 3.45. Nigerlysin is heat stable up to 65 degrees C but unstable at 75 degrees C when incubated for 10min. Circular dichroic analysis revealed that nigerlysin has an alpha helical structure. Exposure of mouse primary cortical neuronal cells to 0.1mugml(-1) of nigerlysin resulted in the rapid loss of their viability, approximately 50% in 24h. The IC(50) is estimated to be 0.037mugml(-1), or between 0.034 and 0.041mugml(-1) at the 95% confidence level. [Description provided by NIOSH]
• Subjects:
  Animals Communicable Diseases Environmental Monitoring Fungi Fungicide, Industrial Hazardous Substances Laboratories Mortality Occupational Diseases Occupational Exposure Occupational Health Safety

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• Keywords:
  Animal-studies Exposure-assessment Exposure-levels Fungicides In-vitro-studies Infectious-diseases Laboratory-animals Mortality-data Mortality-rates Occupational-diseases Occupational-exposure Occupational-hazards Pathogens Toxic-effects Toxins

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• ISSN:
  0300-483X
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Georgia ; Ohio ; OSHA Region 1 ; OSHA Region 3 ; OSHA Region 4 ; OSHA Region 5 ; Rhode Island ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  150-155
• Volume:
  219
• Issue:
  1
• NIOSHTIC Number:
  nn:20029410
• Citation:
  Toxicology 2006 Feb; 219(1-3):150-155
• Email:
  vesper.stephen@epa.gov
• Federal Fiscal Year:
  2006
• NORA Priority Area:
  Research Tools and Approaches: Exposure Assessment Methods
• Peer Reviewed:
  True
• Source Full Name:
  Toxicology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:114e2b479d31c7644136ae6f18170e62fcd14f6338c58b6cc212be887abb231a0aa69c5f82453caad9b44f75632dcf9da91632cd6f93e3ea1667fe4bcc59b4b5
• Download URL:
  https://stacks.cdc.gov/view/cdc/190395/cdc_190395_DS1.pdf
• File Type:
  [PDF - 207.96 KB ]