Use of Double Labeling and Photo CD for Morphometric Analysis of Injured Skeletal Muscle

Details

• Personal Author:
  Gibala MJ ; MacDougall JD ; Miller, G. Roger ; Stauber WT
• Description:
  We used computer-assisted analysis of myofiber cross-sectional areas to measure skeletal muscle responses to injury and disease. We developed a simple, inexpensive method for measuring myofiber size in human muscle samples using Kodak photo compact discs (CDs) as the image source. The photo CD serves as a permanent image storage medium and provides a high-resolution image that can be used to detect small myofibers. The use of double labeling for dystrophin and desmin allowed positive identification of both degenerating and regenerating fibers in a single biopsy specimen. [Description provided by NIOSH]
• Subjects:
  Computers Musculoskeletal Diseases Musculoskeletal System Occupational Health Safety Software
• Keywords:
  Computer Models Diseases Injuries Muscles Musculoskeletal System Disorders Sampling Sampling Methods Skeletal Disorders Skeletal System Disorders
• ISSN:
  0022-1554
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  43
• Issue:
  11
• NIOSHTIC Number:
  nn:20029394
• Citation:
  J Histochem Cytochem 1995 Nov; 43(11):1179-1184
• Contact Point Address:
  William T. Stauber, Ph. D., Department of Physiology, West Virginia University, P.O. Box 9229, Morgantown, WV 26507-9229
• Federal Fiscal Year:
  1996
• Performing Organization:
  West Virginia University, Morgantown, West Virginia
• Peer Reviewed:
  True
• Start Date:
  19920101
• Source Full Name:
  Journal of Histochemistry and Cytochemistry
• End Date:
  20050531
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:d2d17a908f4c8dfd292b9cd8e6f5d514273534791bd943ea525f2e9884143e8a15040cb5aabbfb9a8df940cf2c33fadf66f0674c28347d1698ca84da44e53f03
• Download URL:
  https://stacks.cdc.gov/view/cdc/190382/cdc_190382_DS1.pdf
• File Type:
  [PDF - 8.37 MB ]