Induction of Interleukin-6 by Coal Containing Bioavailable Iron Is Through Both Hydroxyl Radical and Ferryl Species

Details

• Personal Author:
  Huang X ; Zhang Q
• Description:
  Coal mining causes health problems, such as pneumoconiosis. We have previously shown that prevalence of pneumoconiosis in workers from various coalmine regions positively correlates with levels of bioavailable iron (BAI) in the coals from that region. In the present study, the nature of reactive oxygen species formed by BAI in the coals and its mechanisms of the induction of biological responses were investigated. Human lung epithelial cell line, A549 cells, were used to examine the induction of interleukin-6 (IL-6), a pro-inflammatory cytokine, which is known to play a crucial role in the development of pneumoconiosis. We found that levels of IL-6 protein as well as its mRNA were significantly increased in the cells treated for 24 h with 20 microg/cm2 of the BAI-containing Pennsylvania (PA) coal; for example we observed 6.7-fold increase in IL-6 protein. Levels of IL-6 protein in cells treated with the Utah (UT) coal containing low-BAI were only 1.9-fold of the control levels. The enhancing effect on the IL-6 by the PA coal was similar to that caused by hydrogen peroxide. Superoxide dismutase (SOD), catalase (CAT), and N-acetyl-L-cysteine (NAC) all had inhibitory effects on the PA coal-induced IL-6 formation. However, CAT had the least protective effect as compared to SOD and NAC. Our results indicate that BAI in the PA coal may induce IL-6 through both ferryl species (via iron autoxidation) and hydroxyl radicals (via the Fenton/Haber Weiss reactions). [Description provided by NIOSH]
• Subjects:
  Coal Coal Mining Dust Ferrous Compounds Hazardous Substances Lung Lung Diseases Mining Occupational Health Pneumoconiosis Respiratory System Respiratory Tract Diseases Safety

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• Keywords:
  Author Keywords: Coal Black-lung Coal-dust Coal-miners Coal-mining Coal-workers-pneumoconiosis Health-hazards Interleukin-6 Iron Iron-compounds Lung-disease Lung-disorders Occupational-health Oxidative Stress Pulmonary-system-disorders Respiratory-system-disorders

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• ISSN:
  0250-5991
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  New York ; OSHA Region 2
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  95-100
• Volume:
  28
• Issue:
  1
• NIOSHTIC Number:
  nn:20029290
• Citation:
  J Biosci 2003 Feb; 28(1):95-100
• Contact Point Address:
  New York University School of Medicine, Department of Environmental Medicine, 550 First Avenue, PHL Room 802, New York, NY 10016, USA
• Email:
  xihuang@env.med.nyu.edu
• CAS Registry Number:
  Iron (CAS RN 7439-89-6)
• Federal Fiscal Year:
  2003
• Performing Organization:
  New York University Medical Center, New York, New York
• Peer Reviewed:
  True
• Start Date:
  19990930
• Source Full Name:
  Journal of Biosciences
• End Date:
  20030929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:ef4c53154ff06a34df8ec5678e8970b063b0472b45521bb5eeda40dfeb927e1f88772ab78b40a6b87cb0a0427ad9dfd042ec22b1c6ebd071f3386b8837304d11
• Download URL:
  https://stacks.cdc.gov/view/cdc/190314/cdc_190314_DS1.pdf
• File Type:
  [PDF - 161.79 KB ]