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Hyaluronic Acid Binding by Human Sperm Indicates Cellular Maturity, Viability, and Unreacted Acrosomal Status



Details

  • Personal Author:
  • Description:
    To test, both in semen and washed-sperm fractions, whether hyaluronic acid (HA) binding is restricted to sperm that have completed cellular maturation. Comparisons of sperm in semen and in HA-bound sperm fractions. University-based diagnostic and research andrology laboratory. Semen samples originated in men being tested for infertility. The attributes of sperm maturity were tested by immunocytochemistry with creatine kinase and HspA2 antisera (highlights cytoplasmic retention in diminished-maturity sperm), aniline blue chromatin staining (detects persistent histones), pisum sativum lectin staining (reveals acrosomal integrity), and the FertiLight viability kit (highlights viable and nonviable sperm). All markers of sperm maturity and immaturity supported the hypothesis that HA-bound sperm are mature. Nonbinding sperm exhibited cytoplasmic and nuclear properties of diminished maturity. The acrosomal status of HA-bound sperm was either unreacted or slightly capacitated, but not acrosome reacted. Only viable sperm exhibited HA binding. Sperm that are able to bind to HA are mature and have completed the spermiogenetic processes of sperm plasma membrane remodeling, cytoplasmic extrusion, and nuclear histone-protamine replacement. Hyaluronic acid-bound sperm show unreacted acrosomes. These studies provide further insights into the relationship between spermiogenesis and sperm function. [Description provided by NIOSH]
  • Subjects:
  • Keywords:
  • ISSN:
    0015-0282
  • Document Type:
    Text
  • Funding:
    Grant
  • Genre:
    Journal Article
  • Place as Subject:
  • CIO:
    National Institute for Occupational Safety and Health (NIOSH)
  • Topic:
    Workplace Safety & Health
  • Location:
    North America
  • Volume:
    79
  • NIOSHTIC Number:
    nn:20029192
  • Citation:
    Fertil Steril 2003 Jun; 79(Suppl 3):1616-1624
  • Email:
    gabor.huszar@yale.edu
  • Federal Fiscal Year:
    2003
  • Performing Organization:
    Yale University
  • Peer Reviewed:
    True
  • Start Date:
    19990930
  • Source Full Name:
    Fertility and Sterility
  • Supplement:
    3
  • End Date:
    20030929
  • Collection(s):
  • Main Document Checksum:
    urn:sha-512:67828703db950c2ab28717e3a2e1c9e36a0768460b5886fd664ce9dc3e4c548a51e90062dcea6ac5430cc3aca45272fe8aaa03a22038634ad47f93f5f10031ae
  • Download URL:
  • File Type:
    Filetype[PDF - 402.23 KB ]
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