Biochemical Markers of Sperm Function: Male Fertility and Sperm Selection for ICSI

Details

• Personal Author:
  Cayli S ; Celik-Ozenci C ; Delpiano E ; Huszar G ; Jakab A ; Ovari L ; Sakkas D ; Ward D
• Description:
  The expression of a 70 kDa chaperone protein, HspA2 (formerly called CK-M), has been identified in mature human spermatozoa. The central role of HspA2 has been established, as the expression level of this protein is related to sperm cellular maturity, DNA integrity, chromatin maturity, chromosomal aneuploidy frequency and sperm function, including fertilizing potential. The spermiogenetic events of cytoplasmic extrusion and remodelling of the plasma membrane, which facilitate the formation of zona pellucida binding site(s) in human spermatozoa, are related. Finally, the presence of the hyaluronic acid (HA) receptor on the plasma membrane of mature sperm coupled with the HA-coated slide sperm-binding assay, facilitates the testing of infertile men and the selection of single mature spermatozoa for ICSI. Because mature spermatozoa have no residual cytoplasm, the HA-bound sperm fraction is also enriched in spermatozoa that are normal by the Kruger strict morphology method. [Description provided by NIOSH]
• Subjects:
  Biochemistry Occupational Health Safety Sex Characteristics Spermatogenesis Urogenital System
• Keywords:
  Biochemical-indicators Fertility Morphology Sex-factors Sexual-reproduction Spermatozoa
• ISSN:
  1472-6483
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Connecticut ; OSHA Region 1
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  462-468
• Volume:
  7
• Issue:
  4
• NIOSHTIC Number:
  nn:20029152
• Citation:
  Reprod Biomed Online 2003 Oct-Nov; 7(4):462-468
• Contact Point Address:
  The Sperm Physiology and IVF Laboratories, Department of Obstetrics and Gynecology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06510, USA
• Federal Fiscal Year:
  2004
• Performing Organization:
  Yale University
• Peer Reviewed:
  True
• Start Date:
  19990930
• Source Full Name:
  Reproductive Biomedicine Online
• End Date:
  20030929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c68ea26a8a124e1ba1c056b2760f142ba750081a5508e16a62b0bbc123ac56faef6a6a8ae45ac91049e21739374c43390c0801d1fc73f25e1e07ca8b7a27c8e7
• Download URL:
  https://stacks.cdc.gov/view/cdc/190204/cdc_190204_DS1.pdf
• File Type:
  [PDF - 1.15 MB ]