Intracytoplasmic Sperm Injection: A Novel Selection Method for Sperm with Normal Frequency of Chromosomal Aneuploidies

Details

• Personal Author:
  Cayli S ; Delpiano E ; Huszar G ; Jakab A ; Kovanci E ; Ravelli A ; Sakkas D ; Ward D
• Description:
  To test a newly invented intracytoplasmic sperm injection (ICSI) sperm selection method based on sperm hyaluronic acid (HA) binding. Comparison of chromosomal disomy and diploidy frequencies in sperm arising from semen and in HA-bound sperm. Academic andrology laboratory. Men presenting for semen analysis. Washed sperm fractions of 32 semen samples were applied to Petri dishes or glass slides coated with immobilized HA. The unbound sperm were rinsed gently, and the HA-bound sperm were removed with an ICSI pipette. The control sperm population was the unselected sperm. Both HA-selected and unselected sperm were treated with fluorescence in situ hybridization with centromeric probes for the X, Y, and 17 chromosomes. Chromosomal disomy and diploidy frequencies. In the HA-bound sperm (495-2,079 per man, 41,670 in all) compared with unselected sperm (4,770 per man, 162,210 in all), the chromosomal disomy frequencies were reduced to 0.16% from 0.52%, diploidy to 0.09% from 0.51%, and sex chromosome disomy to 0.05% from 0.27% (a 5.4-fold reduction vs. 4-fold respective increase in ICSI offspring). The HA sperm selection method for ICSI, which is based on a relationship between sperm receptors for zona pellucida and HA, will likely reduce the potential genetic complications and adverse public health effects of ICSI. [Description provided by NIOSH]
• Subjects:
  Demography Laboratories Occupational Health Safety Sex Characteristics Spermatogenesis
• Keywords:
  Demographic-characteristics Laboratory-testing Sampling Sampling-methods Sex-factors Spermatozoa
• ISSN:
  0015-0282
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Connecticut ; OSHA Region 1
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  84
• Issue:
  6
• NIOSHTIC Number:
  nn:20029128
• Citation:
  Fertil Steril 2005 Dec; 84(6):1665-1673
• Contact Point Address:
  Gabor Huszar, M.D., Yale School of Medicine, Department of Obstetrics and Gynecology, 333 Cedar Street, New Haven, Connecticut 06520
• Email:
  gabor.huszar@yale.edu
• Federal Fiscal Year:
  2006
• Performing Organization:
  Yale University
• Peer Reviewed:
  True
• Start Date:
  19990930
• Source Full Name:
  Fertility and Sterility
• End Date:
  20030929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:499b926c8e54fddbbbdd19a572fb7a9cd8d68122f9439e0a39b55081e860434e38058e0c126e9f518d3f6f4859657f60408e290b7e3287622ccb1fb149accfc4
• Download URL:
  https://stacks.cdc.gov/view/cdc/190180/cdc_190180_DS1.pdf
• File Type:
  [PDF - 225.96 KB ]