Iron-Induced Interleukin-6 Gene Expression: Possible Mediation Through the Extracellular Signal-Regulated Kinase and p38 Mitogen-Activated Protein Kinase Pathways

Details

• Personal Author:
  Dai J ; Frenkel K ; Huang C ; Huang X ; Wu J ; Yang C
• Description:
  Increased iron store in the body may increase the risk of many diseases such as cancer and inflammation. However, the precise pathogenic mechanism of iron has not yet been elucidated. In the present study, the early biological responses of cells to iron treatment were investigated in AP-1 luciferase reporter stably transfected mouse epidermal JB6 cells and primary rat hepatocytes. It was shown that water-soluble iron compounds, such as FeSO4 and Fe2(SO4)3, were more active in inducing AP-1 in JB6 cells than water-insoluble iron compounds, such as Fe2O3 and FeS. Iron stimulated mitogen-activated protein kinase (MAPK) family members of extracellular signal-regulated kinases (ERKs) and p38 MAPK but not c-jun NH2 terminal kinases (JNKs), both in JB6 cells and in primary rat hepatocytes, as determined by the phosphorylation assay. Interestingly, the increase in AP-1 luciferase activity by iron was inhibited by the pretreatment of the cells with PD98059, a specific MEK1 inhibitor, and SB202190, a p38 kinase inhibitor. Levels of interleukin-6 (IL-6), a pro-inflammatory cytokine, were increased in JB6 cells by iron in a dose-dependent manner. The increase in IL-6 and its mRNA by iron was also eliminated by the pretreatment of the cells with PD98059 and SB202190. Since the IL-6 promoter contains an AP-1 binding site, our studies indicate that the iron-induced IL-6 gene expression may be mediated through ERKs and p38 MAPK pathways, possibly one of the important mechanisms for the pathogenesis of iron overload. [Description provided by NIOSH]
• Subjects:
  Calcium Compounds Coal Coal Mining Dust Ferrous Compounds Hazardous Substances Lung Lung Diseases Mining Occupational Health Respiratory System Respiratory Tract Diseases Safety

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• Keywords:
  Black-lung Calcium-compounds Cell-damage Coal-dust Coal-miners Coal-mining Coal-workers-pneumoconiosis Health-hazards Injuries Injury-prevention Iron-compounds Lung-cells Lung-disease Pneumoconiosis

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• ISSN:
  0300-483X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  New York ; OSHA Region 2
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  199-209
• Volume:
  203
• Issue:
  1
• NIOSHTIC Number:
  nn:20029043
• Citation:
  Toxicology 2004 Oct; 203(1-3):199-209
• Contact Point Address:
  Department of Environmental Medicine, NYU Cancer Institute, New York University School of Medicine, PHL Room 802, New York, NY 10016
• CAS Registry Number:
  Calcium (CAS RN 7440-70-2) ; Calcium carbonate (CAS RN 471-34-1) ; Iron (CAS RN 7439-89-6)
• Federal Fiscal Year:
  2005
• Performing Organization:
  New York University Medical Center, New York, New York
• Peer Reviewed:
  True
• Start Date:
  19990930
• Source Full Name:
  Toxicology
• End Date:
  20030929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7e23d58cddba295dcab684161680f75547fcf975710be5a63913b38a25ca16b5b913cc2b64c3257aac397cb5427f65b9ba6541169e681b582e32b373609452c8
• Download URL:
  https://stacks.cdc.gov/view/cdc/190115/cdc_190115_DS1.pdf
• File Type:
  [PDF - 274.83 KB ]