Cross-Reactivity of Monoclonal Antibodies Against Aspergillus versicolor and Other Fungi and Their Implications for the Development of Antibody-Based Monitoring Techniques for Fungi

Details

• Personal Author:
  Lewis, Daniel M. ; Schmechel D ; Simpson J
• Description:
  In order to improve current culture-based monitoring techniques for Aspergillus versicolor and other fungi in general, our laboratory is developing species-specific monoclonal antibody (mAb)-based immunoassay techniques. In this study we attempted to enhance the production of mAbs specific for A. versicolor by immunizing mice with pre-formed immune complexes to block epitopes of spores known to be cross-reactive with other fungi. We found that immunization with immune complexes did not enhance the production of A. versicolor-specific mAbs. While the antibody reactivity to related Aspergillus and Penicillium species was reduced, the response to other fungi was enhanced. The patterns of cross-reactivity among the 50 tested fungal species not only demonstrate that phylogenetically distant fungi share common epitopes but also that different fungi express a given cross-reactive epitope in variable amounts on a per spore basis. [Description provided by NIOSH]
• Subjects:
  Animals Antibodies Fungi Fungicide, Industrial Laboratories Occupational Health Safety
• Keywords:
  Animal-studies Antibody-response Fungicides Laboratory-animals Laboratory-techniques Monitoring-systems
• ISBN:
  9780970991515
• Publisher:
  Fungal Research Group Foundation, Inc.
• Document Type:
  Text
• Genre:
  Proceedings
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  366-374
• NIOSHTIC Number:
  nn:20029014
• Citation:
  Bioaerosols, fungi, bacteria, mycotoxins and human health: patho-physiology, clinical effects, exposure assessment, prevention and control in indoor environments and work. Johanning E, ed., Albany, NY: Fungal Research Group Foundation, Inc., 2005 Mar; :366-374
• Contact Point Address:
  National Institute for Occupational Safety and Health, Health Effects Laboratory Division, 1095 Willowdale Road, M/S H-4020, Morgantown, WV 26505, USA
• Email:
  dschmechel@cdc.gov
• Editor(s):
  Johanning E
• Federal Fiscal Year:
  2005
• NORA Priority Area:
  Research Tools and Approaches: Exposure Assessment Methods
• Peer Reviewed:
  False
• Source Full Name:
  Bioaerosols, fungi, bacteria, mycotoxins and human health: patho-physiology, clinical effects, exposure assessment, prevention and control in indoor environments and work
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0adf30f2994478a01f3ae7542ca6604604792b98949d11e9c26556a0a6f8e0d681fe24a4520b4f8f618553bca296b728597e69e2cf77357cefaab330aae2f030
• Download URL:
  https://stacks.cdc.gov/view/cdc/190091/cdc_190091_DS1.pdf
• File Type:
  [PDF - 1.11 MB ]