Comparison of Established Cell Lines at Different Passages by Karyotype and Comparative Genomic Hybridization

Details

• Personal Author:
  Bairwa N ; Bamezai R ; Grant SG ; Sargent LM ; Senft JR ; Wenger SL
• Description:
  Two established cancer cell lines, MCF-7 and Ishikawa, were both obtained directly from a cell repository and through another laboratory. The karyotypes from the two MCF-7 cell lines had up to 83 chromosomes and similarities for chromosomal gain and structural abnormalities. The two Ishikawa cell lines had up to 60 chromosomes with only a missing X as the common chromosome abnormality. CGH studies were performed by co-hybridizing the two Ishikawa or MCF-7 cell lines to normal metaphases. The differences seen between the two MCF-7 cell cultures reflect changes due to passage number and culture conditions. For Ishikawa, DNA polymorphic data and mutation studies suggest that the two cell lines are not derived from the same established tumor cell line. Our study shows the utilization of CGH in comparing cell lines originating from the same specimen. Our study also demonstrates the necessity for periodically evaluating cell lines to confirm their origin. [Description provided by NIOSH]
• Subjects:
  Cells, Cultured Chromosomes Laboratories Neoplasms Occupational Health Safety
• Keywords:
  Analytical-processes Cell-culture-techniques Cell-cultures Chromosome-damage Laboratory-testing Tumors
• ISSN:
  0144-8463
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  24
• Issue:
  6
• NIOSHTIC Number:
  nn:20028396
• Citation:
  Biosci Rep 2004 Dec; 24(6):631-639
• Contact Point Address:
  Department of Pathology, West Virginia University, P. O. Box 9203, Morgantown, WV, 26506-9203
• Email:
  swenger@hsc.wvu.edu
• Federal Fiscal Year:
  2005
• Peer Reviewed:
  True
• Source Full Name:
  Bioscience Reports
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:2a97244c57e0bc20e0d64c7b256342636c055931a3f2006fb74c4807e7f32341d06abeddcb274047e653e3a61e9af72793fa10d5cac9768fe4af237f1f747a49
• Download URL:
  https://stacks.cdc.gov/view/cdc/189847/cdc_189847_DS1.pdf
• File Type:
  [PDF - 1.80 MB ]