Arsenite Modulation of the Integrity of Actin Filaments and the Increase in Cell Motility May Be Mediated Through CDC42

Details

• Personal Author:
  Flynn DC ; Qian Y ; Shi X ; Zhang Z
• Description:
  Arsenite is a human carcinogen, however, the molecular mechanisms involved are poorly understood. By stimulating human endothelial cells (SVEC4-10) with arsenite, we found that arsenite modulated the integrity of actin filaments, and stimulated the formation of cell motility organelle structures within 5 minutes. Further, we found arsenite increased the cell motility, indicating that structural changes in cell morphology induced by arsenite are relevant to cellular functions. Affinity absorption assays with GST-PAK-PBD demonstrated that arsenite activated small GTPase CDC42 and Rac. We also observed that arsenite induced the formation of hygrogen radicals in SVEC4-10 cells, which are known to exert a regulatory role in arsenite induced effects on SVEC4-10 cells. Taken together, our data demonstrate that arsenite increases cell motility, which may be involved in cancer matastasis. [Description provided by NIOSH]
• Subjects:
  Carcinogens Occupational Health Safety
• Keywords:
  Arsenites Carcinogenesis Carcinogenicity Cell-morphology
• ISSN:
  0892-6638
• Document Type:
  Text
• Genre:
  Proceedings ; Abstract
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  16
• Issue:
  4
• NIOSHTIC Number:
  nn:20028371
• Citation:
  FASEB J 2002 Mar; 16(4)(Pt I):A171
• Contact Point Address:
  PPRB, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505
• CAS Registry Number:
  Arsenite (CAS RN 15502-74-6)
• Federal Fiscal Year:
  2002
• NORA Priority Area:
  Research Tools and Approaches: Cancer Research Methods
• Peer Reviewed:
  False
• Part Number:
  I
• Source Full Name:
  The FASEB Journal
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:6b0bd0e236a11b0067de3ef47f91d30d88fdcbf8d6f908bd61d0eee25328a53c726f01fc73333927c550c4fc1e49493774faee9ed723cc29d5dff78ab40097e4
• Download URL:
  https://stacks.cdc.gov/view/cdc/189842/cdc_189842_DS1.pdf
• File Type:
  [PDF - 987.51 KB ]