2D and 3D Assessment of Neuropathology in Rat Brain After Prenatal Exposure to Methylazoxymethanol, a Model for Developmental Neurotoxicity

Details

• Personal Author:
  Bos-Kuijpers MHM ; DeGroot DMG ; Gundersen HG ; Hartgring S ; Kaufmann WSH ; Lammers JHCM ; Moerkens M ; O'Callaghan JP ; Otto M ; Pakkenberg B ; Van de Horst L ; Waalkens-Berendsen IDH
• Description:
  To evaluate the ability of a tiered quantitative morphological approach to reveal developmental neurotoxicity, morphometric parameters were measured in the offspring of rats treated with methylazoxymethanol (MAM) during days 13-15 of pregnancy. Treatment was aimed at inhibiting the proliferation phase of hippocampal neurons while leaving cerebellar neurons unaffected. 2D and 3D assessment of brain morphology combined with straightforward measurement of brain size, weight and volume, and the usefulness of estimation of total neuron numbers were studied. Each tier indicated major effects of MAM, from macroscopic effects in the cerebrum (first tier) to a considerable loss of neurons in the hippocampal CA1 pyramidal layer (third tier). The cerebellum and the number of cerebellar granular neurons were not changed. Along with each step of the proposed tiered approach (brain size -> linear morphometry -> stereology), the discriminative strength of the endpoints, and thus the probability to pinpoint the extent and location of developmental brain lesions increased. [Description provided by NIOSH]
• Subjects:
  Animals Hazardous Substances Laboratories Neoplasms Nervous System Nervous System Diseases Occupational Health Safety
• Keywords:
  Animal-studies Brain-damage Brain-disorders Brain-tumors Laboratory-animals Morphology Neurotoxic-effects Neurotoxicity Neurotoxicology Neurotoxins Quantitative-analysis Toxic-effects Toxic-materials Toxins

  More +
• ISSN:
  0890-6238
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  417-432
• Volume:
  20
• Issue:
  3
• NIOSHTIC Number:
  nn:20028144
• Citation:
  Reprod Toxicol 2005 Sep/Oct; 20(3):417-432
• Contact Point Address:
  de Groot DMG, TNO, Qual Life, Utrechtseweg 48,POB 360, NL-3700 AJ Zeist, Netherlands
• Federal Fiscal Year:
  2005
• Peer Reviewed:
  True
• Source Full Name:
  Reproductive Toxicology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:138bf3c2918f56895732bf7a64eed762733af9e3789b557be92108c54f1f1baf57cec0c2104e7e44e2532ba80ed54118f2cdc922a241597c44520e9faa9c6339
• Download URL:
  https://stacks.cdc.gov/view/cdc/189793/cdc_189793_DS1.pdf
• File Type:
  [PDF - 927.55 KB ]