Cytokine and Growth Factor Gene Expression in Response to Trimethyl Tin (TMT)-Induced Neurotoxicity in the Adult Rat Hippocampus

Details

• Personal Author:
  Little AR ; O'Callaghan JP
• Description:
  Cytokines and growth factors are proposed to play a role in the homeostasis of the central nervous system (CNS) and its response to injury and disease. A role for these pleiotropic mediators in the early activation of microgial and astrocytic reactions to neural injury constitutes a dominant theme in contemporary neuroscience literature, despite the fact that most data are derived from in vitro preparations. Understanding the earliest events associated with toxicant-induced brain injury is necessary to detect and modify CNS damage following injury or disease. Here we examined the in vivo expression of interleukin-1 alpha (IL-1alpha), interleukin-6 (IL-6), transforming growth factor-beta (TGF-beta), brain-derived neurotrophic factor (BDNF), and nerve growth factor (NGF) using the TMT model of neuronal injury (8mg/kg i.p.) using semi-quantitative Reverse Transcription-Polymerase Chain Reaction (RT-PCR) at 24 hours post TMT. This timepoint was chosen based on reports in the literature that mRNA for TGF-beta and IL-6 are up-regulated at this time with other types of nerve injury. No significant changes for BDNF, NGF, or IL-1alpha were seen. mRNA for TGF-beta and IL-6 were increased slightly less than 2 fold, which is a very small increase, but is consistent with published reports. These results suggest that these neuroimmune/neurotrophic mediators do not play a crucial role in the activation of microglia and astrocytes following toxicant-induced injury to the CNS. Additional toxicants need to be examined in order to determine the generality of these observations to the neurotoxic condition. [Description provided by NIOSH]
• Subjects:
  Animals Chemistry Chemistry, Analytic Growth Hazardous Substances Laboratories Nervous System Occupational Health Safety Toxicology
• Keywords:
  Analytical-chemistry Analytical-methods Animal-studies Central-nervous-system Chemical-analysis Growth-factors Laboratory-animals Nerve-damage Nervous-system Neurological-system Neurotoxic-effects Neurotoxicity Neurotoxicology Neurotoxins Statistical-analysis Toxic-materials Toxins

  More +
• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  Louisiana ; OSHA Region 3 ; OSHA Region 6 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  239-240
• Volume:
  48
• NIOSHTIC Number:
  nn:20028011
• Citation:
  Toxicologist 1999 Mar; 48(1-S):239-240
• CAS Registry Number:
  Trimethylstannane (CAS RN 1631-73-8)
• Federal Fiscal Year:
  1999
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 38th Annual Meeting, March 14-18, 1999, New Orleans, Louisiana
• Supplement:
  1-S
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:153814fedb8a62b724df93edb055f3bedae9bfb1c860363d7662f83de6470ab910746c45f55fc70912badfc1268ce11ad50c0c128cb06883d69b6bed2bbf7623
• Download URL:
  https://stacks.cdc.gov/view/cdc/189727/cdc_189727_DS1.pdf
• File Type:
  [PDF - 370.51 KB ]