Mutagenicity of N-OH-MOCA (4-Amino-4′-Hydroxylamino-Bis-3,3′-Dichlorodiphenylmethane) and PBQ (2-Phenyl-1,4-Benzoquinone) in Human Lymphoblastoid Cells

Details

• Personal Author:
  Cheever KL ; DeBord, D. Gayle ; Reid TM ; Savage RE Jr.
• Description:
  The genotoxic potential of two occupationally significant chemicals, 4,4'-methylene-bis-2-chloroaniline (MOCA) and 2-phenyl-1,4-benzoquinone (PBQ), was explored by monitoring the induction of mutations at the HPRT locus of AHH-1 human lymphoblastoid cells. Exposure of AHH-1 cells to the putative carcinogenic metabolite of MOCA, N-OH-MOCA, induced a 6-fold increase in mutant frequency and resulted in base pair substitutions primarily at A:T base pairs. In contrast, exposure to PBQ did not result in an increased mutant frequency although this compound was significantly more cytotoxic than N-OH-MOCA at equimolar doses. The induction of mutations at A:T sites by N-OH-MOCA is consistent with the type of DNA damage known to be produced by MOCA and provides a specific marker of genotoxic damage for exposed populations. [Description provided by NIOSH]
• Subjects:
  Environmental Monitoring Mutagens Occupational Health Safety
• Keywords:
  Exposure-assessment Genotoxic-effects Mutagenesis Mutagenicity
• ISSN:
  0378-4274
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DBBS - Division of Biomedical and Behavioral Sciences
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  205-210
• Volume:
  95
• Issue:
  3
• NIOSHTIC Number:
  nn:20027936
• Citation:
  Toxicol Lett 1998 May; 95(3):205-210
• Contact Point Address:
  Division of Biomedical and Behavioral Sciences, National Institute for Occupational Safety and Health, 4676 Columbia Parkway, Cincinnati, OH 45226, USA
• Email:
  tar9@cdc.gov
• Federal Fiscal Year:
  1998
• Peer Reviewed:
  True
• Source Full Name:
  Toxicology Letters
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:93d9fa806fdec656d55cc3f7782dd2a20e184319111aca450c2709adb333c7a85c3f0ed7e96081b4c599ec7f29cebb494c49cb02b755fa7876087ef0de2a47e4
• Download URL:
  https://stacks.cdc.gov/view/cdc/189682/cdc_189682_DS1.pdf
• File Type:
  [PDF - 81.40 KB ]