Detection of Polycyclic Aromatic Hydrocarbon-DNA Adducts in Placental DNA Samples by Room-Temperature Solid-Matrix Phosphorescence

Details

• Personal Author:
  Hurtubise RJ ; Manchester DK ; Thompson AL ; Weston A
• Description:
  This is the first attempt for the direct detection of polycyclic aromatic hydrocarbon (PAH)-DNA adducts in human placental DNA samples by solid-matrix phosphorescence (SMP). Six samples were investigated, and SMP emission spectra and the corresponding second derivative SMP spectra were obtained for all the samples. Numerous excitation and emission wavelengths were studied for detecting PAH-DNA adducts. Second derivative SMP spectra indicated the presence of PAH-DNA adducts, whereas the longer SMP emission region proved fruitful for detecting adducts in the placental DNA samples. The SMP results for the samples strongly implied that a variety of PAH-DNA adducts could be present. [Description provided by NIOSH]
• Subjects:
  Hydrocarbons Occupational Health Phosphorous Compounds Polycyclic Aromatic Hydrocarbons Safety
• Keywords:
  DNA-adducts Phosphorous-compounds Polycyclic-aromatic-hydrocarbons Polycyclic-hydrocarbons Sample-preparation Sampling-methods
• ISSN:
  0003-2719
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Colorado ; OSHA Region 3 ; OSHA Region 8 ; Utah ; West Virginia ; Wyoming
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  41
• Issue:
  11
• NIOSHTIC Number:
  nn:20034353
• Citation:
  Anal Lett 2008 Jan; 41(11):1944-1963
• Contact Point Address:
  Robert J. Hurtubise, University of Wyoming, Department of Chemistry, Laramie, WY 82071
• Email:
  hurtubis@uwyo.edu
• Federal Fiscal Year:
  2008
• Peer Reviewed:
  True
• Source Full Name:
  Analytical Letters
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:b0f1adc4fb3d5c6dcf5d271084307698f8138da373734deb67d3d5dfee7797aa2c3b8f887d5d7b48887ab57362c157dbe3c2ea52363e9c41c5796773dde0a16a
• Download URL:
  https://stacks.cdc.gov/view/cdc/189590/cdc_189590_DS1.pdf
• File Type:
  [PDF - 389.67 KB ]