Unrestrained Acoustic Plethysmograph for Measuring Specific Airway Resistance in Mice

Details

• Personal Author:
  Frazer DG ; Johnson VJ ; Reynolds JS
• Description:
  An acoustic whole body plethysmograph was developed to estimate specific airway resistance (sRaw) in unrestrained mice. The plethysmograph uses acoustic principles to measure the thoracic breathing pattern and simultaneously measures the airflow entering and/or leaving the plethysmograph. Similarly to traditional methods utilizing a double-chamber plethysmograph, these measurements were combined to estimate sRaw. To evaluate the new system, we placed six conscious A/J mice individually in a whole body plethysmograph (Buxco System) for a 2-min exposure to aerosolized methacholine chloride dissolved in saline (0, 5, 10, and 20 mg/ml), which is known to increase sRaw in mice. Three minutes after exposure, the mice were transferred to the acoustic plethysmograph for 2 min for data collection. The mean baseline value of sRaw was 0.93+/-0.10 cmH2O.s. A dose-dependent increase in sRaw was shown, with an approximate tripling of sRaw at the highest dose. These results demonstrate the ability of the system to estimate sRaw based on plethysmograph airflow and acoustic amplitude. [Description provided by NIOSH]
• Subjects:
  Acoustics Air Movements Animals Laboratories Lung Noise Occupational Health Respiratory System Safety
• Keywords:
  Acoustic-absorption Acoustic-trauma Acoustical-measurements Air-flow Animal-studies Breathing Laboratory-animals Lung-burden Lung-function Plethysmographs Plethysmography Statistical-analysis

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• ISSN:
  8750-7587
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  105
• Issue:
  2
• NIOSHTIC Number:
  nn:20034350
• Citation:
  J Appl Physiol 2008 Aug; 105(2):711-717
• Contact Point Address:
  J. S. Reynolds, Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road - ms L2101, Morgantown, WV 26505, USA
• Email:
  jsr0@cdc.gov
• Federal Fiscal Year:
  2008
• NORA Priority Area:
  Construction ; Manufacturing
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Applied Physiology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:95bf835b0e32a8c9564c0cf1459b91f67f669dbeda296e64c9d9a1ce2f8526a011e99c380a478e6a2044aacab0e4d99dfabe34ce2aed59217807d9b56d380c11
• Download URL:
  https://stacks.cdc.gov/view/cdc/189588/cdc_189588_DS1.pdf
• File Type:
  [PDF - 426.79 KB ]