Differential Biocide Susceptibility of the Multiple Genotypes of Mycobacterium immunogenum

Details

• Personal Author:
  Khan IU ; Selvaraju SB ; Yadav JS
• Description:
  The non-tuberculous mycobacterium Mycobacterium immunogenum colonizes industrial metalworking fluids (MWFs) presumably due to its relative resistance to the currently practiced biocides and has been implicated in occupational respiratory hazards, particularly hypersensitivity pneumonitis. With an aim to understand its inherent biocide susceptibility profile and survival potential in MWF, five different genotypes of this organism, including a reference genotype (700506) and four novel test genotypes (MJY-3, MJY-4, MJY-10 and MJY-12) isolated in our recent study from diverse MWF operations were evaluated. For this, two commercial biocide formulations, Grotan (Hexahydro-1,3,5-tris(2-hydroxyethyl)-s-triazine) and Kathon (5-chloro-2-methyl-4-isothiazolin-3-one) currently practiced for the control of microorganisms, including mycobacteria, in MWF operations were tested. Effect of the fluid matrix on the biocide susceptibility was investigated for the synthetic (S) and semi-synthetic (SS) MWF matrices. In general, the minimum inhibitory concentration values were higher for the HCHO-releasing biocide Grotan than the isothiazolone biocide Kathon. All genotypes (except the reference genotype) showed lower susceptibility in SS as compared to S fluid matrix for Grotan. However, in case of Kathon, a greater susceptibility was observed in SS fluid for majority of the test genotypes (MJY-3, 4 and 10). The test genotypes were more resistant than the reference genotype to either biocide in both fluid types. Furthermore, the individual genotypes showed differential biocidal susceptibility, with MJY-10 being the most resistant. These observations emphasize the importance of using the resistant genotypes of M. immunogenum as the test strains for formulation or development and evaluation of existing and novel biocides, for industrial applications. [Description provided by NIOSH]
• Subjects:
  Hazardous Substances Metals Metal Workers Microbiology Occupational Diseases Occupational Exposure Occupational Health Respiratory Tract Diseases Safety
• Keywords:
  Bacterial-cultures Metalworking Metalworking-fluids Metalworking-industry Microscopic-analysis Occupational-diseases Occupational-exposure Occupational-health Respiratory-system-disorders Toxic-materials
• ISSN:
  1367-5435
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  197-203
• Volume:
  35
• Issue:
  3
• NIOSHTIC Number:
  nn:20033888
• Citation:
  J Ind Microbiol Biotechnol 2008 Mar; 35(3):197-203
• Contact Point Address:
  Jagjit S. Yadav, Department of Environmental Health, University of Cincinnati College of Medicine, Cincinnati, OH, 45267-0056
• Email:
  Jagjit.Yadav@uc.edu
• Federal Fiscal Year:
  2008
• Performing Organization:
  University of Cincinnati
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Journal of Industrial Microbiology and Biotechnology
• End Date:
  20170731
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0361855dd866fa3d13685bea77a94d900ec1ed95db63d64a2fec12cb5cb6ea9776b3128e1c56351158415ef7df58d69e326454bdca639df22694d81e7a1c4a56
• Download URL:
  https://stacks.cdc.gov/view/cdc/189299/cdc_189299_DS1.pdf
• File Type:
  [PDF - 583.58 KB ]