Sequential Exposure to Carbon Nanotubes and Bacteria Enhances Pulmonary Inflammation and Infectivity

Details

• Personal Author:
  Antonini JM ; Barchowsky A ; Castranova, Vincent ; Fabisiak JP ; Feng WH ; Kagan VE ; Kisin ER ; Kommineni C ; Murray AR ; Reynolds J ; Roberts, Jennifer R. ; Shvedova AA ; Tyurina YY ; Antonini JM ; Barchowsky A ; Castranova, Vincent ; Fabisiak JP ; Feng WH ; Kagan VE ; Kisin ER ; Kommineni C ; Murray AR ; Reynolds J ; Roberts, Jennifer R. ; Shvedova AA ; Tyurina YY
• Description:
  Carbon nanotubes (CNT), with their applications in industry and medicine, may lead to new risks to human health. CNT induce a robust pulmonary inflammation and oxidative stress in rodents. Realistic exposures to CNT may occur in conjunction with other pathogenic impacts (microbial infections) and trigger enhanced responses. We evaluated interactions between pharyngeal aspiration of single-walled CNT (SWCNT) and bacterial pulmonary infection of C57BL/6 mice with Listeria monocytogenes (LM). Mice were given SWCNT (0, 10, and 40 mug/mouse) and 3 days later were exposed to LM (10(3) bacteria/mouse). Sequential exposure to SWCNT/LM amplified lung inflammation and collagen formation. Despite this robust inflammatory response, SWCNT pre-exposure significantly decreased the pulmonary clearance of LM-exposed mice measured 3 to 7 days after microbial infection versus PBS/LM-treated mice. Decreased bacterial clearance in SWCNT-pre-exposed mice was associated with decreased phagocytosis of bacteria by macrophages and a decrease in nitric oxide production by these phagocytes. Pre-incubation of naïve alveolar macrophages with SWCNT in vitro also resulted in decreased nitric oxide generation and suppressed phagocytizing activity toward LM. Failure of SWCNT-exposed mice to clear LM led to a continued elevation in nearly all major chemokines and acute phase cytokines into the later course of infection. In SWCNT/LM-exposed mice, bronchoalveolar lavage neutrophils, alveolar macrophages, and lymphocytes, as well as lactate dehydrogenase level, were increased compared with mice exposed to SWCNT or LM alone. In conclusion, enhanced acute inflammation and pulmonary injury with delayed bacterial clearance after SWCNT exposure may lead to increased susceptibility to lung infection in exposed populations. [Description provided by NIOSH]
• Subjects:
  Animals Dust Lung Occupational Health Particulate Matter Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Alveolar-cells Animal-studies Fibrogenesis Nanotechnology Particulate-dust Pulmonary-system-disorders Respirable-dust Respiratory-system-disorders Vitamins
• ISSN:
  1044-1549
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article ; Journal Article
• Place as Subject:
  OSHA Region 3 ; Pennsylvania ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH) ; National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health ; Workplace Safety & Health
• Location:
  North America ; North America
• Volume:
  38
• Issue:
  5
• NIOSHTIC Number:
  nn:20033862
• Citation:
  Am J Respir Cell Mol Biol 2008 May; 38(5):579-590
• Contact Point Address:
  Anna A. Shvedova, Ph.D., Pathology/Physiology Research Branch, HELD, National Institute for Occupational Safety and Health, 1095 Willowdale Rd., Morgantown, WV 26505
• Email:
  ats1@cdc.gov
• Federal Fiscal Year:
  2008
• NORA Priority Area:
  Manufacturing ; Manufacturing
• Performing Organization:
  University of Pittsburgh at Pittsburgh
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  American Journal of Respiratory Cell and Molecular Biology
• End Date:
  20160630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:21a57eb9aee48c28b4005e3e8d9b1b2bcc25362f420abf3945971ccf84f9cda4ce55f8b2de9503f18dc722f983708d6da6079d23347ed5efb3f5f31711478c2a
• Download URL:
  https://stacks.cdc.gov/view/cdc/189278/cdc_189278_DS1.pdf
• File Type:
  [PDF - 1.35 MB ]