Assessment of Autofluorescent Alveolar Macrophages and Centriacinar Emphysema as Markers of Smoking Status in Decreased Coal Miners

Details

• Personal Author:
  Battelli LA ; Ducatman BS ; Fekedulegn D ; Hubbs, Ann F. ; Kashon ML ; Keshava N ; Rao GVS ; Vallyathan V ; Vi LH ; Battelli LA ; Ducatman BS ; Fekedulegn D ; Hubbs, Ann F. ; Kashon ML ; Keshava N ; Rao GVS ; Vallyathan V ; Vi LH
• Description:
  Background: smoking status is often not known in autopsy studies Potential surrogates for this information include severity of centriacinar emphysema (CE) and autofluorescent macrophages. Cigarette smoke contains many gaseous and particulate components, and a strong intracellular fluorescence of these components is present in unfixed alveolar macrophages from smokers and ex-smokers, but has not been studied in paraffin-embedded tissue. Design: using lung tissue from 100 random cases in the National Coal Workers' Autopsy Study, we reviewed the intensity (qualitative, 0-2+) and alveolar area fraction of autofluorescent macrophages (AAFAM) in deparaffinized, unstained sections at three different wavelengths (blue, ultraviolet (DAPI)) at 360-370 nm excitation, green (FITC) at 460-500 nm excitation, and red (Texas RED) at 532.5-5875 nm excitation. Quantitative studies utilized a commercial image analysis system (Melamorph(tradename) Imaging System, Universal Imaging Corporation). In addition, the severity of CE (0-2+) was evaluated by 2 blinded observers, but focal emphysema (associated with coal workers' pneumoconiosis) was not scored as part of this. These results were compared to the next-of-kin reported smoking status using SAS statistical software. Results: CE and smoking status were not significantly associated (p=-O2658) in these miners. Qualitative assessment of autofluorescence intensity correlated with smoking status, as did AAFAM at 532.5-5g7.5 nm excitation (p
• Subjects:
  Coal Coal Mining Dust Lung Lung Diseases Mining Occupational Health Particulate Matter Respiratory System Respiratory Tract Diseases Safety Smokers

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• Keywords:
  Alveolar-cells Coal-dust Coal-miners Coal-workers Lung-cells Lung-disorders Lung-irritants Particulate-dust Particulates Pulmonary-disorders Pulmonary-system Smoking

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• ISSN:
  0893-3952
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings ; Abstract or Summary ; Proceedings
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH) ; National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health ; Workplace Safety & Health
• Location:
  North America ; North America
• Volume:
  16
• Issue:
  1
• NIOSHTIC Number:
  nn:20033686
• Citation:
  Mod Pathol 2003 Jan; 16(1):8A
• Federal Fiscal Year:
  2003
• Peer Reviewed:
  False
• Source Full Name:
  Modern Pathology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:b9c1e1bbc1a44dce176ee27cafd4cfc4f5eb4e91f5fe377840e47970f806060268eb94237ecbb1246f8308c00118213fbc8f52899cb323d838641ed3bf27a480
• Download URL:
  https://stacks.cdc.gov/view/cdc/189165/cdc_189165_DS1.pdf
• File Type:
  [PDF - 139.30 KB ]