Direct Detection of Pb in Urine and CD, Pb, Cu, and Ag in Natural Waters Using Electrochemical Sensors Immobilized with DMSA Functionalized Magnetic Nanoparticles

Details

• Personal Author:
  Addleman RS ; Choi D ; Fryxell GE ; Hongsirikarn K ; Sangvanich T ; Timchalk C ; Toloczko MB ; Warner CL ; Warner MG ; Yantasee W
• Description:
  Urine is universally recognized as one of the best non-invasive matrices for biomonitoring exposure to a broad range of xenobiotics, including toxic metals. Detection of metal ions in urine has been problematic due to the protein competition and electrode fouling. For direct, simple, and field-deployable monitoring of urinary Pb, electrochemical sensors employing superparamagnetic iron oxide (Fe3O4) nanoparticles with a surface functionalization of dimercaptosuccinic acid (DMSA) has been developed. The metal detection involves rapid collection of dispersed metal-bound nanoparticles from a sample solution at a magnetic or electromagnetic electrode, followed by the stripping voltammetry of the metal in acidic medium. The sensors were evaluated as a function of solution pH, the binding affinity of Pb to DMSA-Fe3O4, the ratio of nanoparticles per sample volume, preconcentration time, and Pb concentrations. The effect of binding competitions between the DMSA-Fe3O4 and urine constituents for Pb on the sensor responses was studied. After 90 s of preconcentration in samples containing 25 vol.% of rat urine and 0.1 g L-1 of DMSA-Fe3O4, the sensor could detect background level of Pb (0.5 ppb) and yielded linear responses from 0 to 50 ppb of Pb, excellent reproducibility (%RSD of 5.3 for seven measurements of 30 ppb Pb), and Pb concentrations comparable to those measured by ICP-MS. The sensor could also simultaneously detect background levels (<1 ppb) of Cd, Pb, Cu, and Ag in river and seawater. [Description provided by NIOSH]
• Subjects:
  Acids Biomarkers Hazardous Substances Metals Metals, Heavy Occupational Health Safety Urine
• Keywords:
  Acidity Analytical-instruments Analytical-processes Detectors Electrochemistry Heavy-metals Magnetic-properties Metal-compounds Metallic-ions Nanotechnology Toxic-materials Urinalysis Urine-chemistry

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• ISSN:
  0003-2654
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 10 ; Washington
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  348-355
• Volume:
  133
• Issue:
  3
• NIOSHTIC Number:
  nn:20033304
• Citation:
  Analyst 2008 Mar; 133(3):348-355
• Contact Point Address:
  Pacific Northwest National Laboratory (PNNL), Richland, WA 99352
• Email:
  wassana.yantasee@pnl.gov
• CAS Registry Number:
  Cadmium (CAS RN 7440-43-9) ; Copper (CAS RN 7440-50-8) ; Ferrous oxide (CAS RN 1345-25-1) ; Lead (CAS RN 7439-92-1) ; Mercury (CAS RN 7439-97-6) ; Silver (CAS RN 7440-22-4)
• Federal Fiscal Year:
  2008
• Performing Organization:
  Battelle Pacific Northwest Laboratories
• Peer Reviewed:
  True
• Start Date:
  20060901
• Source Full Name:
  Analyst
• End Date:
  20090831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:6cba5d658927e66be4b6811df009ae806f27ba38e6838f81a34378ded8fb7c1de88d61d64b1a40326c65900c531fc07ec677a990d4f904f2d96ac4d58e342321
• Download URL:
  https://stacks.cdc.gov/view/cdc/188938/cdc_188938_DS1.pdf
• File Type:
  [PDF - 236.08 KB ]