Development and Validation of a Fecal Testosterone Biomarker in Mus musculus and Peromyscus maniculatus

Details

• Personal Author:
  Billitti JE ; Lasley BL ; Wilson BW
• Description:
  This is a report on the development and validation of an ELISA method to determine fecal testosterone levels, and on their evaluation as a biomarker for adverse effects of endocrine-disrupting chemicals on reproductive health using male rodents of the Peromyscus maniculatus and Mus musculus species as an animal model. The ELISA antibody had the highest specificity for testosterone (100%), followed by dihydrotestosterone (57.4%) and androstenediol (0.27%). Radiolabeled testosterone was injected i.p. into three mice. Fecal samples were collected, extracted, and analyzed by liquid scintillation counting. The ELISA was performed to characterize the excretion kinetics and metabolic fate of circulating testosterone. Solubilization of feces with 10% methanol overnight provided an extraction efficiency of 87% for all metabolites; an ethyl ether extraction was more selective for testosterone. The fecal excretion of the testosterone was a biphasic process with a majority of the radioactivity recovered in the first 24 hours. HPLC analysis revealed at least five testosterone metabolites in feces, with most metabolites being less polar than testosterone. This study forms the initial evaluation of what will become a field monitoring tool. [Description provided by NIOSH]
• Subjects:
  Animals Biomarkers Endocrine System Endocrine System Diseases Energy Metabolism Occupational Health Safety Urogenital Diseases Urogenital System
• Keywords:
  Animal-studies Endocrine-function Endocrine-system Endocrine-system-disorders Endocrinology Hormone-activity Hormones Kinetic-energy Kinetics Metabolic-disorders Metabolic-rate Metabolic-study Metabolism Reproduction Reproductive-effects Reproductive-hazards Reproductive-system Reproductive-system-disorders

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• ISSN:
  0006-3363
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  59
• Issue:
  5
• NIOSHTIC Number:
  nn:20033102
• Citation:
  Biol Reprod 1998 Nov; 59(5):1023-1028
• Contact Point Address:
  Barry W. Wilson, Department of Environmental Toxicology, University of California at Davis, Davis, California 95616
• Federal Fiscal Year:
  1999
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  True
• Start Date:
  19900930
• Source Full Name:
  Biology of Reproduction
• End Date:
  20020929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:085051d26f936f335351b5d9e0bbb6156a1e69cbece98fc7440edbe089985d59f4d4419315b00cbb425819f2928a8763edd6457230bc2819eefb106dd05083ab
• Download URL:
  https://stacks.cdc.gov/view/cdc/188805/cdc_188805_DS1.pdf
• File Type:
  [PDF - 144.47 KB ]