Unusual Peroxidase Activity of Polynitroxylated Pegylated Hemoglobin: Elimination of H(2)O(2) Coupled with Intramolecular Oxidation of Nitroxides

Details

• Personal Author:
  Bayr H ; Hsia CJC ; Huang Z ; Kagan VE ; Kapralov A ; Kochanek PM ; Ma L ; Maeda A ; Osipov A ; Stoyanovsky DA
• Description:
  Polynitroxylated hemoglobin (Hb(AcTPO)(12)) has been developed as a hemoglobin-based oxygen carrier. While Hb(AcTPO)(12) has been shown to exert beneficial effects in a number of models of oxidative injury, its peroxidase activity has not been characterized thus far. In the blood stream, Hb(AcTPO)(12) undergoes reduction by ascorbate to its hydroxylamine form Hb(AcTPOH)(12). Here we report that Hb(AcTPOH)(12) exhibits peroxidase activity where H(2)O(2) is utilized for intramolecular oxidation of its TPOH residues to TPO. This represents an unusual redox-catalytic mechanism whereby reduction of H(2)O(2) is achieved at the expense of reducing equivalents of ascorbate converted into those of Hb(AcTPOH)(12), a new propensity that cannot be directly associated with ascorbate. [Description provided by NIOSH]
• Subjects:
  Blood Hematologic Tests Microbiology Occupational Health Safety
• Keywords:
  Author Keywords: Hemoglobin Biological-effects Blood-analysis Blood-cells Blood-serum Blood-stream Catalysis Cell-biology Cell-damage Cell-function Cellular-reactions H2O2 Hydroxylation-reactions Microscopic-analysis Molecular-biology Molecular-structure Nitroxide Oxidation Oxidation-reduction-reactions Oxidative-processes Oxygen-transport Peroxidase Plasma-membrane

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• ISSN:
  0006-291X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  California ; Georgia ; OSHA Region 3 ; OSHA Region 4 ; OSHA Region 9 ; Pennsylvania ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  139-143
• Volume:
  399
• Issue:
  2
• NIOSHTIC Number:
  nn:20037617
• Citation:
  Biochem Biophys Res Commun 2010 Aug; 399(2):139-143
• Contact Point Address:
  Valerian E. Kagan, Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh, PA 15219
• Email:
  kagan@pitt.edu
• Federal Fiscal Year:
  2010
• NORA Priority Area:
  Manufacturing
• Performing Organization:
  University of Pittsburgh at Pittsburgh
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Biochemical and Biophysical Research Communications
• End Date:
  20160630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:d0cf60957caa1fb7a42763cda3e4021d6179ab89e6c8b895c145a2f720209f79a4f6225268fe9041a03a8fb6037ce5b67c688c39a082b33f6e2bbc4bccca83bf
• Download URL:
  https://stacks.cdc.gov/view/cdc/188680/cdc_188680_DS1.pdf
• File Type:
  [PDF - 434.53 KB ]