Development of an HPLC Method for Simultaneous Analysis of Five Antineoplastic Agents

Details

• Personal Author:
  Dillon HK ; Khazaeli MB ; Larson RR
• Description:
  Simultaneous analysis of common antineoplastic agents potentially hazardous to healthcare workers is of much interest for the evaluation of the overall health risk to these workers. Such analysis could be applied to both air and surface monitoring samples to provide a broader indication of risk to combinations of these agents. It was determined that the ability to simultaneously evaluate five frequently used, potentially hazardous agents was sufficient for general evaluation of exposures to healthcare workers. The approach used to select the five agents was to obtain a list of the agents used most frequently in both a cancer hospital and an outpatient cancer treatment center, then review the list to determine which agents were potentially more hazardous to human health. From these reviews, it was decided to attempt to develop an analytical method able to detect and quantify the presence of 5-fluorouracil, ifosfamide, cyclophosphamide, doxorubicin HCl, and paclitaxel. A reverse-phase high performance liquid chromatograph (HPLC) with a Waters Symmetry C8 column and a UV wavelength of 195 nm was selected for method development. The mobile phase was 22.75 percent acetonitrile in water buffered to a pH of 6.0. The HPLC analytical method developed is able to detect all five agents of interest, and at minimum detectable concentrations of 0.5-microg/mL for each of the five agents. [Description provided by NIOSH]
• Subjects:
  Antineoplastic Agents Hazardous Substances Health Care Facilities Workforce And Services Health Personnel Occupational Exposure Occupational Health Safety
• Keywords:
  Analysis Antineoplastic-agents Author Keywords: Antineoplastics Cyclophosphamide Doxorubicin Drugs Fluorouracil Hazardous-materials Health-care-facilities Health-care-personnel Health-hazards HPLC Ifosfamide Occupational-hazards Paclitaxel

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• ISSN:
  1047-322X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Alabama ; OSHA Region 4 ; OSHA Region 8 ; Utah
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  109-119
• Volume:
  18
• Issue:
  2
• NIOSHTIC Number:
  nn:20037544
• Citation:
  Appl Occup Environ Hyg 2003 Feb; 18(2):109-119
• CAS Registry Number:
  5-Fluorouracil (CAS RN 51-21-8) ; Cyclophosphamide (CAS RN 50-18-0) ; Doxorubicin (CAS RN 23214-92-8) ; Ifosfamide (CAS RN 3778-73-2) ; Paclitaxel (CAS RN 33069-62-4)
• Federal Fiscal Year:
  2003
• Performing Organization:
  University of Utah
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Applied Occupational and Environmental Hygiene
• End Date:
  20280630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:d288b5da0b5155d3aa7a7d307a31dcfd6b4cf8fbf25a18cc7ddbeaa9499cc8de9f37e0f71c48207692998ddcd3b8a91e1a838da0173d3cd90f8e4870c396b89a
• Download URL:
  https://stacks.cdc.gov/view/cdc/188621/cdc_188621_DS1.pdf
• File Type:
  [PDF - 477.31 KB ]