Calcium and Calmodulin Stimulated In Vitro Phosphorylation of Rooster Brain Tubulin and MAP-2 Following a Single Oral Dose of Tri-O-Cresyl Phosphate

Details

• Personal Author:
  Abou-Donia MB ; Lapadula DM ; Suwita E
• Description:
  The effect of a single oral administration of 750 mg/kg tri-o-cresyl phosphate (TOCP) on endogenous phosphorylation of specific brain cytosolic proteins has been studied in roosters following the development of delayed neurotoxicity. In vitro phosphorylation assay using [Y-32P]ATP was carried out. Proteins were then resolved on one dimensional 8% SDS-PAGE and two-dimensional gel electrophoresis, stained with Coomassie blue and autoradiographed. The amount of proteins, as well as the amount of 32P incorporation, were quantified by microdensitometry, TOCP-administration enhanced the phosphorylation of cytosolic proteins of Mr 52-59 kDa, 70 kDa, and 300 kDa by as much as 155%, 199% and 166%, respectively. Two-dimensional gel electrophoresis confirmed the 52-59 kDa proteins as a and ß tubulin and the 300 kDa protein as microtubule-associated protein-2. [Description provided by NIOSH]
• Subjects:
  Animals Environmental Monitoring Laboratories Nervous System Nervous System Diseases Occupational Health Phosphoric Acids Safety
• Keywords:
  Animal-studies Author Keywords: Tri-o-cresyl Phosphate (TOCP) Brain-damage Brain-function Central-nervous-system Exposure-assessment Exposure-levels Laboratory-animals Microtubule-associated Protein-2 (MAP-2) Phosphorylation Neurotoxic-effects Neurotoxicity Phosphates Protein-biochemistry Tubulin Phosphorylation

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• ISSN:
  0006-8993
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  North Carolina ; OSHA Region 4
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  199-203
• Volume:
  374
• Issue:
  1
• NIOSHTIC Number:
  nn:20037444
• Citation:
  Brain Res 1986 May; 374(1):199-203
• CAS Registry Number:
  Calcium (CAS RN 7440-70-2)
• Federal Fiscal Year:
  1986
• NORA Priority Area:
  Neurotoxic Effects
• Performing Organization:
  Duke University, Durham, North Carolina
• Peer Reviewed:
  True
• Start Date:
  19840601
• Source Full Name:
  Brain Research
• End Date:
  19881031
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0aee652be85db0649a0f30504e4f997f2fe2bb743b7284b5e7933dddb9cbb597d73242ad93fb9d44d36ebf8a5a3e7bd1ede71ccaf5dd49dcbed5a02f5dda6aee
• Download URL:
  https://stacks.cdc.gov/view/cdc/188540/cdc_188540_DS1.pdf
• File Type:
  [PDF - 446.08 KB ]