Inhibition of Quantitative PCR Analysis of Fungal Conidia Associated with Indoor Air Particulate Matter

Details

• Personal Author:
  Lees PSJ ; McDevitt JJ ; Merz WG ; Schwab KJ
• Description:
  Environmental samples analyzed by quantitative PCR (qPCR) are subject to interference by inhibitors present in the environment being sampled. A controlled determination of the effect of inhibitors associated with sampling indoor air and the ability of internal standard controls to detect inhibition was carried out by filter collection of air samples followed by spiking of the filters with green fluorescent protein-expressing Aspergillus fumigatus conidia. Microscopic conidial counts were compared with qPCR results and correlated with levels of particulate matter and viable airborne microorganisms. Our data showed that PCR can be inhibited by masses of particulate matter as low as 50 µg and that the amount of inhibition was positively correlated with the mass of particulate (r = 0.75) and the number of non-filamentous organisms (r = 0.73). The use of internal standard DNA identified the presence of inhibitors and indicated the need for additional sample processing or qualification of sample results. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Environmental Health Environmental Monitoring Fungi Microbiology Occupational Health Particulate Matter Safety
• Keywords:
  Air-samples Airborne-particles Analytical-methods Analytical-processes Aspergillus Fumigatus Author Keywords: Airborne Fungi Conidia Environmental-factors Microorganisms Particulate-matter Particulate-sampling-methods Particulates Quantitative PCR Sample Inhibition Sampling Sampling-methods

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• ISSN:
  0393-5965
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Maryland ; Massachusetts ; OSHA Region 1 ; OSHA Region 3
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  35-45
• Volume:
  23
• Issue:
  1
• NIOSHTIC Number:
  nn:20037416
• Citation:
  Aerobiologia 2007 Jan; 23(1):35-45
• Contact Point Address:
  K. J. Schwab, Department of Environmental Health Sciences, The Johns Hopkins University, Bloomberg School of Public Health, 615 N. Wolfe St., Baltimore, MD 21205, USA
• Email:
  kschwab@jhsph.edu
• Federal Fiscal Year:
  2007
• Performing Organization:
  Johns Hopkins University
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Aerobiologia
• End Date:
  20280630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:9969bbdee594e3ff97ac520d03331d66b883388dbbb28320c5f38326a5c82e864c29f8b89e7c8cfca11e2910cffd0bd219fa4056e9a8cb84db3b2810ff78957f
• Download URL:
  https://stacks.cdc.gov/view/cdc/188514/cdc_188514_DS1.pdf
• File Type:
  [PDF - 281.88 KB ]