Evaluation of Gene Expression Through qRT-PCR in Cyclically Loaded Tendons: An In Vivo Model

Details

• Personal Author:
  Asundi KR ; King KB ; Rempel DM ; Asundi KR ; King KB ; Rempel DM
• Description:
  An in vivo rabbit animal model for the tendinopathy, epicondylitis, was used to examine the effects of repetitive load on the expression of various genes associated with matrix remodeling. Following 80 h of cumulative load, tissue from the distal and proximal regions of the flexor digitorum profundus tendon was collected. Quantitative RT-PCR was used to asses mRNA levels of collagenase-1 (MMP-1), stromelysin (MMP-3), vascular endothelial growth factor (VEGF), connective tissue growth factor (CTGF), cyclooxygenase-2 (COX-2), interleukin-1β (IL-1β), type III collagen (COL-III) and fibronectin (FBRN). No significant differences in expression levels were found between loaded and unloaded limbs at either region of the tendon. The findings were unexpected as the same model has already demonstrated an increase in the density of cells staining for VEGF and CTGF. Different regulatory mechanisms between mRNA and protein expression or localized changes missed due to homogenization of the tissue samples, may explain the discrepancy in findings. [Description provided by NIOSH]
• Subjects:
  Biomechanical Phenomena Cumulative Trauma Disorders Environmental Monitoring Ergonomics Genetics Laboratories Musculoskeletal Diseases Musculoskeletal System Nervous System Occupational Health Risk Assessment Risk Factors Safety

  More +
• Keywords:
  Author Keywords: Overuse Injury Biological-effects Biological-function Biomechanical-engineering Biomechanical-modeling Biomechanics Cell-biology Cellular-reactions Connective Tissue Growth Factor Cumulative-trauma Cumulative-trauma-disorders Exposure-assessment Exposure-levels Exposure-methods Genetic-factors Laboratory-testing Mechanics Musculoskeletal-system Musculoskeletal-system-disorders Neurological-reactions Physiological-effects Physiological-measurements Physiological-response Quantitative-analysis Quantitative RT-PCR Repetitive-work Risk-analysis Risk-factors Tissue-disorders Tissue-distribution Vascular Endothelial Growth Factor

  More +
• ISSN:
  1439-6319
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article ; Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH) ; National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health ; Workplace Safety & Health
• Location:
  North America ; North America
• Pages in Document:
  265-270
• Volume:
  102
• Issue:
  3
• NIOSHTIC Number:
  nn:20037281
• Citation:
  Eur J Appl Physiol 2008 Mar; 102(3):265-270
• Contact Point Address:
  D. M. Rempel, Ergonomics Program, 1301 South 46th Street, Building 163, Richmond, CA 94804
• Email:
  david.rempel@ucsf.edu
• Federal Fiscal Year:
  2008
• Performing Organization:
  University of California, Berkeley
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  European Journal of Applied Physiology
• End Date:
  20250630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a582a691b0adfd22baa3f0063c1db336bf8d36f15c7cdc1ddeb833e32427dd185b8bf38eca9db7dc794b74ec032311e2a3ead865b19231e244319243a832cbdc
• Download URL:
  https://stacks.cdc.gov/view/cdc/188397/cdc_188397_DS1.pdf
• File Type:
  [PDF - 317.56 KB ]