Cytokine and Pharmacological Regulation of Lung Fibroblast Proliferation

Details

• Personal Author:
  Blackford J ; Castranova, Vincent ; Dey R ; Reist RH ; Vrana K
• Description:
  Our objective was to study the role of macrophage-derived cytokines in fibroblast proliferation. [3H] thymdine incorporation was shown to correlate well with fibroblast proliferation. In vitro treatment of alveolar macrophages with silica (150microg/ml) resulted in the production of an agent(s) which inhibits fibroblast proliferation. Indomethacin, a prostaglandin synthetase inhibitor, reversed this inhibition and unmasked a previously unseen competence factor(s). Further, the proliferative action of exogenous PDGF was reduced when fibroblasts were treated with supernate from silica-exposed macrophages, presumably due to the inhibitory action of prostaglandins. Silica-induced pulmonary fibrosis may result from decreased release of prostaglandins and/or increased release of proliferative factors from alveolar macrophages. Indeed, macrophages harvested 40 days after intratracheal instillation of silica (42mg/rat) produced an agent(s) which significantly stimulated [3H] thymidine incorporation by pulmonary fibroblasts. The antifibrotic drug, tetrandrine, inhibited [3H] thymidine incorporation and fibroblast proliferation in response to either serum or PDGF plus plasma. Thus, tetrandrine may be a useful probe to study fibrogenesis. (BOM-5431) [Description provided by NIOSH]
• Subjects:
  Fibrosis Lung Lung Diseases Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Cell-function Cellular-function Lung-disease Lung-disorders Lung-function Lung-tissue Pulmonary-function Pulmonary-system Pulmonary-system-disorders Respiratory-system-disorders
• ISSN:
  0731-9193
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  Florida ; OSHA Region 3 ; OSHA Region 4 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  12
• Issue:
  1
• NIOSHTIC Number:
  nn:20037144
• Citation:
  Toxicologist 1992 Feb; 12(1):390
• Federal Fiscal Year:
  1992
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 31st Annual Meeting, February 23-27,1992, Seattle, Washingtion
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:93b99094af93dd78c08a2a012a60760b189a18a504b07df72d99624696e7640312ffc7d2703e36cf14779ef2dbc02361618627b09c4a57e52f84a9756c31f728
• Download URL:
  https://stacks.cdc.gov/view/cdc/188293/cdc_188293_DS1.pdf
• File Type:
  [PDF - 89.73 KB ]