Histone Deacetylase and Acetyltransferase Inhibitors Decrease Organic Dust-Mediated Activation of Proinflammatory Gene Expression

Details

• Personal Author:
  LeVan TD ; Romberger DJ ; Weiler Z
• Description:
  RATIONALE: Post-translational modification of histone proteins are major mechanisms that modify chromatin structure and regulate gene expression in eukaryotes. Activation of histone acetyltransferase (HAT) or inhibition of histone deacetylases (HDAC) is generally believed to allow chromatin to assume a more open state, permitting transcriptional activity. METHODS: Organic dust was obtained from settled surface dust from swine confinement facilities and extracted in Hanks' balanced salt solution. Bronchial epithelial cells, BEAS-2B cell line, were pretreated for 30-120 min with trichostatin A (1-10 ug/ml) and anacardic acid (0.1 to 1 uM), then stimulated with organic dust for 4 hours. Cell-free supernatants were harvested and assayed for IL-6 and IL-8 by ELISA. RESULTS: We report here the surprising observation that treatment of a human bronchial epithelial cell line, BEAS-2B cells, with the inhibitor histone deacetylase, trichostatin A, or the histone acetyltransferase inhibitor, anacardic acid, strongly inhibited induction of both IL-6 and IL-8 protein upon stimulation with organic dust. CONCLUSION: These data suggest that chromatin remodeling regulates organic dust-induced IL-6 and IL-8 protein production. The mechanism (s) of this regulation will be an area of future investigation. [Description provided by NIOSH]
• Subjects:
  Agriculture Air Pollutants, Occupational Asthma Asthma, Occupational Dust Environmental Monitoring Genetics Inhalation Exposure Irritants Lung Lung Diseases Occupational Diseases Occupational Exposure Occupational Health Particulate Matter Respiratory Hypersensitivity Respiratory System Respiratory Tract Diseases Risk Assessment Risk Factors Safety Workplace

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• Keywords:
  Agricultural-industry Agricultural-processes Agricultural-workers Airborne-particles Biological-effects Biological-monitoring Breathing Bronchial-asthma Cell-transformation Cellular-reactions Dust-exposure Dust-inhalation Dust-measurement Dust-particles Exposure-assessment Exposure-levels Exposure-methods Genes Genetic-factors Inhalants Inhalation-studies Lung-cells Lung-disease Lung-disorders Lung-irritants Occupational-diseases Occupational-exposure Occupational-hazards Occupational-health Occupational-respiratory-disease Organic-dusts Particulate-dust Pulmonary-disorders Pulmonary-system Pulmonary-system-disorders Respirable-dust Respiratory-hypersensitivity Respiratory-irritants Respiratory-rate Respiratory-system-disorders Risk-analysis Risk-factors Statistical-analysis Treatment Work-areas Work-environment Work-operations Work-performance Work-practices Workplace-studies

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• ISSN:
  1073-449X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  Nebraska ; OSHA Region 7
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  181
• NIOSHTIC Number:
  nn:20037110
• Citation:
  Am J Respir Crit Care Med 2010 May; 181(Meeting Abstracts):A2010
• Federal Fiscal Year:
  2010
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of Nebraska Medical Center, Omaha, Nebraska
• Peer Reviewed:
  False
• Start Date:
  20060801
• Source Full Name:
  American Journal of Respiratory and Critical Care Medicine
• Supplement:
  Meeting Abstracts
• End Date:
  20160731
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:118b2524747c442275e8017c52e734da9241efe47d9efe649d5270c7371fad8b28b615ab73851d2f76d58c3568d05e525183af9cd9f1fdf7e3229026ef610be5
• Download URL:
  https://stacks.cdc.gov/view/cdc/188262/cdc_188262_DS1.pdf
• File Type:
  [PDF - 16.39 KB ]