Inertial Microfluidics for Sheath-Less High-Throughput Flow Cytometry

Details

• Personal Author:
  Bhagat AA ; Kaval N ; Kuntaegowdanahalli SS ; Papautsky I ; Seliskar CJ
• Description:
  Flow cytometer is a powerful single cell analysis tool that allows multi-parametric study of suspended cells. Most commercial flow cytometers available today are bulky, expensive instruments requiring high maintenance costs and specially trained personnel for operation. Hence, there is a need to develop a low cost, portable alternative that will aid in making this powerful research tool more accessible. In this paper we describe a sheath-less, on-chip flow cytometry system based on the principle of Dean coupled inertial microfluidics. The design takes advantage of the Dean drag and inertial lift forces acting on particles flowing through a spiral microchannel to focus them in 3-D at a single position across the microchannel cross-section. Unlike the previously reported micro-flow cytometers, the developed system relies entirely on the microchannel geometry for particle focusing, eliminating the need for complex microchannel designs and additional microfluidic plumbing associated with sheath-based techniques. In this work, a 10-loop spiral microchannel 100 µm wide and 50 µm high was used to focus 6 µm particles in 3-D. The focused particle stream was detected with a laser induced fluorescence (LIF) setup. The microfluidic system was shown to have a high throughput of 2,100 particles/sec. Finally, the viability of the developed technique for cell counting was demonstrated using SH-SY5Y neuroblastoma cells. The passive focusing principle and the planar nature of the described design will permit easy integration with existing lab-on-a-chip (LOC) systems. [Description provided by NIOSH]
• Subjects:
  Laboratories Occupational Health Particulate Matter Safety
• Keywords:
  Author Keywords: Microfluidics Cell-function Cell Counting Cellular-function Flow Cytometry Fluid-mechanics Fluids Laboratory-equipment Laboratory-testing Particle-aerodynamics Particulates

  More +
• ISSN:
  1387-2176
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  187-195
• Volume:
  12
• Issue:
  2
• NIOSHTIC Number:
  nn:20037015
• Citation:
  Biomed Microdevices 2010 Apr; 12(2):187-195
• Contact Point Address:
  Ian Papautsky, Department of Electrical and Computer Engineering, University of Cincinnati, 814 Rhodes Hall, ML030, Cincinnati, OH 45221, USA
• Email:
  ian.papautsky@uc.edu
• Federal Fiscal Year:
  2010
• Performing Organization:
  University of Cincinnati
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Biomedical Microdevices
• End Date:
  20260630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:dcf88e7ae9e05c9badca6fe8a7ce0bdf0a06be759f70480cce1c9b34eae4eddab246a142543e41a62a1a1f7c7e4ed4a9c0414874f3ee9906ad2f107eec8805af
• Download URL:
  https://stacks.cdc.gov/view/cdc/188193/cdc_188193_DS1.pdf
• File Type:
  [PDF - 513.13 KB ]