Evaluation of a Procedure for the Simultaneous Quantification of 4-Ketocyclophosphamide, Cyclophosphamide, and Ifosfamide in Human Urine

Details

• Personal Author:
  B'Hymer, Clayton ; Cheever KL
• Description:
  An accurate and precise analysis procedure is presented for the detection and quantification of cyclophosphamide (CP), 4- ketocyclophosphamide (4-keto-CP), a primary metabolite of CP, and ifosfamide (IF) in human urine. CP and IF are common antineoplastic drugs used for the treatment of many types of cancer. Workers in the healthcare field, including nurses and pharmacists who interact with or prepare prescriptions for patients, have potential low-level exposure to the parent drugs; therefore, an analysis procedure is needed. The main focus of this procedure is the quantitation of 4-keto-CP because it is a primary metabolite of CP exposure and stable under physiological conditions. Sample preparation consists of liquid-liquid extraction of urine with ethyl acetate, and the analysis consists of reversed-phase highperformance liquid chromatography coupled with tandem mass spectrometry for detection of the analytes. Accuracy and precision of this procedure is demonstrated by means of recovery experiments. Recoveries are between 97-1 05 % of theory for the three target analytes at various concentrations (25, 50, 100, and 375 ng/mL for 4-keto-CP; 1, 2, 4, and 15 ng/mL for CP and IF) with relative standard deviations of 8.4% or less. The limit of detection for this procedure is 1 ng/mL for 4-keto-CP, 0.1 nglmL for CP, and 0.05 nglmL for IF in urine. [Description provided by NIOSH]
• Subjects:
  Biochemistry Environmental Monitoring Hazardous Substances Health Personnel Occupational Health Safety Urine Urogenital System
• Keywords:
  Biochemical-analysis Biodynamics Biohazards Biological-effects Biological-systems Drug-interaction Exposure-assessment Exposure-levels Exposure-methods Health-care-personnel Humans Medical-personnel Medicinal-chemicals Physiological-chemistry Physiological-factors Physiological-response Quantitative-analysis Urine-chemistry Urogenital-system

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• ISSN:
  0021-9665
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  328-333
• Volume:
  48
• Issue:
  5
• NIOSHTIC Number:
  nn:20036840
• Citation:
  J Chromatogr Sci 2010 May-Jun; 48(5):328-333
• Contact Point Address:
  C. B'Hymer, U.S. DHHS, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Division of Applied Research and Technology, Taft Laboratory C-23, 4676 Columbia Parkway, Cincinnati, OH 45226
• Federal Fiscal Year:
  2010
• NORA Priority Area:
  Healthcare and Social Assistance ; Services
• Peer Reviewed:
  False
• Source Full Name:
  Journal of Chromatographic Science
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:9bb71eeeeb5104023c867a5e84110689570118128f6521309d0f2601bdb07bf77009c26f3ddf5ed06537c2436fe3f91656fee92bfa594522d815b4ec207d2a3e
• Download URL:
  https://stacks.cdc.gov/view/cdc/188094/cdc_188094_DS1.pdf
• File Type:
  [PDF - 358.91 KB ]