In Vitro Lymphocyte Responses in Contact Hypersensitivity III

Details

• Personal Author:
  Milner JE
• Description:
  Guinea pigs were sensitized to one of two contact allergens: dinitrofluorobenzene (DNFH) or paraphenylenediamine (PDA). Sensitization was accomplished by footpad injections of the hapten conjugated to an extract of soluble guinea pig epidermal proteins in Freund's complete adjuvant. Lymphocytes from animals with contact hypersensitivity to DNFB or PDA were cultured in vitro with guinea pig epidermal proteins conjugated to DNFB or PDA. Responses to these antigens, as measured by incorporation of tritiated thymidine into DNA, indicated that lymphocyte transformation in vitro can distinguish between sensitivity to either of these allergens. [Description provided by NIOSH]
• Subjects:
  Antigens Dermatitis Environmental Monitoring Hypersensitivity Immune System Laboratories Occupational Health Safety Skin Skin Diseases
• Keywords:
  Allergic-dermatitis Allergic-disorders Allergic-reactions Allergies Dermatosis Exposure-assessment Exposure-levels Exposure-methods Immunology Laboratory-animals Laboratory-testing Skin-disorders Skin-exposure Skin-irritants Skin-sensitivity

  More +
• ISSN:
  0022-202X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 10 ; Washington
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  388-391
• Volume:
  58
• Issue:
  6
• NIOSHTIC Number:
  nn:20036830
• Citation:
  J Invest Dermatol 1972 Jun; 58(6):388-391
• Contact Point Address:
  John E Milner, Department of Medicine, School of Medicine, and the Department of Environmental Health, School of Public Health, University of Washington, Seattle, Washington, 98195
• Federal Fiscal Year:
  1972
• NORA Priority Area:
  Disease and Injury: Allergic and Irritant Dermatitis
• Performing Organization:
  University of Washington, Seattle, Washington
• Peer Reviewed:
  True
• Start Date:
  19710501
• Source Full Name:
  Journal of Investigative Dermatology
• End Date:
  19790630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:dd394384ab9e60be39e37df81ba89094e55306c8378d2272c3e23d181132299d38e1c3d27500157adf261e341f9a571332f665c8d76d6eb1f3abdd4beed03fba
• Download URL:
  https://stacks.cdc.gov/view/cdc/188085/cdc_188085_DS1.pdf
• File Type:
  [PDF - 1.35 MB ]