Muramic Acid, Endotoxin, 3-Hydroxy Fatty Acids, and Ergosterol Content Explain Monocyte and Epithelial Cell Inflammatory Responses to Agricultural Dusts

Details

• Personal Author:
  Bailey KL ; Burrell AM ; Dooley GP ; Mehaffy J ; Poole JA ; Reynolds SJ ; Romberger DJ ; Saito, Rena
• Description:
  In agricultural and other environments, inhalation of airborne microorganisms is linked to respiratory disease development. Bacterial endotoxins, peptidoglycans, and fungi are potential causative agents, but relative microbial characterization and inflammatory comparisons amongst agricultural dusts are not well described. The aim of this study was to determine the distribution of microbial endotoxin, 3-hydroxy fatty acids (3-OHFA), muramic acid, and ergosterol and evaluate inflammatory responses in human monocytes and bronchial epithelial cells with various dust samples. Settled surface dust was obtained from five environments: swine facility, dairy barn, grain elevator, domestic home (no pets), and domestic home with dog. Endotoxin concentration was determined by recombinant factor C (rFC). 3-OHFA, muramic acid, and ergosterol were measured using gas chromatography-mass spectrometry. Dust-induced inflammatory cytokine secretion in human monocytes and bronchial epithelial cells was evaluated. Endotoxin-independent dust-induced inflammatory responses were evaluated. Endotoxin and 3-OHFA levels were highest in agricultural dusts. Muramic acid, endotoxin, 3-OHFA, and ergosterol were detected in dusts samples. Muramic acid was highest in animal farming dusts. Ergosterol was most significant in grain elevator dust. Agricultural dusts induced monocyte tumor necrosis factor (TNF) alpha, interleukin (IL)-6, IL-8, and epithelial cell IL-6 and IL-8 secretion. Monocyte and epithelial IL-6 and IL-8 secretion was not dependent on endotoxin. House dust(s) induced monocyte TNFalpha, IL-6, and IL-8 secretion. Swine facility dust generally produced elevated responses compared to other dusts. Agricultural dusts are complex with significant microbial component contribution. Large animal farming dust(s)-induced inflammation is not entirely dependent on endotoxin. Addition of muramic acid to endotoxin in large animal farming environment monitoring is warranted. [Description provided by NIOSH]
• Subjects:
  Agriculture Bacteria Dust Endotoxins Microbiology Occupational Health Particulate Matter Respiratory System Safety
• Keywords:
  Agricultural Industry Bacterial Dusts Dust Exposure Dust Particles Exposure Assessment Inhalation Studies Microscopic Analysis Particulate Dust Particulates
• ISSN:
  1528-7394
• Document Type:
  Text
• Funding:
  Cooperative Agreement ; Grant
• Genre:
  Journal Article
• Place as Subject:
  Colorado ; Nebraska ; OSHA Region 7 ; OSHA Region 8
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  73
• Issue:
  10
• NIOSHTIC Number:
  nn:20036719
• Citation:
  J Toxicol Environ Health A 2010 Jan; 73(10):684-700
• Contact Point Address:
  Jill A. Poole, MD, Pulmonary, Critical Care, Sleep & Allergy Section, University of Nebraska Medical Center, 985300 The Nebraska Medical Center, Omaha, NE 68198-5300, USA
• Email:
  japoole@unmc.edu
• Federal Fiscal Year:
  2010
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  Colorado State University - Ft. Collins
• Peer Reviewed:
  True
• Start Date:
  20030915
• Source Full Name:
  Journal of Toxicology and Environmental Health, Part A: Current Issues
• End Date:
  20270914
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:9912f2c933e42125a90162089088681cbdc233af63d85dc00987ed72b99fc48ec902ae554a030a2bd88e591f693b99913ca5acaeb0a324ef0419eb7840f932e1
• Download URL:
  https://stacks.cdc.gov/view/cdc/188029/cdc_188029_DS1.pdf
• File Type:
  [PDF - 427.51 KB ]