A Paired Comparison Between Human Skin and Hairless Guinea Pig Skin In Vitro Permeability and Lag Time Measurements for 6 Industrial Chemicals

Details

• Personal Author:
  Barbero AM ; Frasch, H. Fredrick
• Description:
  The purpose of the present study was to measure and compare permeability coefficients (k(p)) and lag times (T) in human skin and hairless guinea pig (HGP) skin. Paired experiments employed heat-separated epidermal membranes from human and HGP sources mounted on static in vitro diffusion cells. Infinite-dose, saturated aqueous solutions of 6 industrial chemicals were used as donors: aniline, benzene, 1,2-dichloroethane, diethyl phthalate, naphthalene, and tetrachloroethylene. No significant differences were found between human and HGP skin for either k(p) or tau for any of these chemicals (p >= .24). HGP vs. human k(p) measurements, and HGP vs. human T measurements, were highly correlated. For k(p') the slope of the linear correlation was close to unity (1.080 +/- 0.182) and the intercept close to 0 (0.015 +/- 4029cm/h), with a correlation coefficient (r(2)) = 0.898. For T, the slope was also close to unity (0.818 +/- 0.030) and the intercept close to 0 (-0.014 +/- 0.023 h), with r(2) = 0.994. These results suggest that HGP skin may serve as an excellent surrogate for human skin in in vitro dermal penetration studies. [Description provided by NIOSH]
• Subjects:
  Animals Chemistry Environmental Monitoring Laboratories Occupational Health Safety Skin Skin Absorption
• Keywords:
  1,2-dichloroethane Aniline Animal-studies Author Keywords: Skin Absorption Benzene Biological-effects Biological-monitoring Chemical-deposition Chemical-properties Chemical-reactions Diethyl Phthalate Exposure-assessment Exposure-levels Exposure-methods Humans Laboratory-animals Lag Time Naphthalene Permeability Skin-absorption Skin-exposure Skin-irritants Skin-sensitivity Statistical-analysis Tetrachloroethylene

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• ISSN:
  1556-9527
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 3 ; OSHA Region 5 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  107-113
• Volume:
  28
• Issue:
  3
• NIOSHTIC Number:
  nn:20036039
• Citation:
  Cutan Ocul Toxicol 2009 Sep; 28(3):107-113
• Contact Point Address:
  H. Frederick Frasch, CDC, NIOSH, HELD, MS L-3030, 1095 Willowdale Road, Morgantown, West Virginia 26505
• Email:
  hbf9@cdc.gov
• CAS Registry Number:
  1,2-Dichloroethane (CAS RN 107-06-2) ; Aniline (CAS RN 62-53-3) ; Benzene (CAS RN 71-43-2) ; Naphthalene (CAS RN 91-20-3) ; Tetrachloroethylene (CAS RN 127-18-4)
• Federal Fiscal Year:
  2009
• NORA Priority Area:
  Manufacturing ; Services
• Peer Reviewed:
  True
• Source Full Name:
  Cutaneous and Ocular Toxicology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:d0c1b7a5f310b289d1e9506a1fc1eb515bb52289da9da48f9b29999737da454460a180b433d069045c3e16ba580a55a19bcbd615d56c1cdda75c09c32db3582b
• Download URL:
  https://stacks.cdc.gov/view/cdc/187647/cdc_187647_DS1.pdf
• File Type:
  [PDF - 972.44 KB ]