Comparative Effects of Quinoline and 8-Hydroxyquinoline on Glutathione Levels, DNA Fragmentation and Enzyme Induction in Cultured Rat Hepatocytes

Details

• Personal Author:
  Plews PI ; Snawder, John E. ; Tirmenstein MA ; Toraason M
• Description:
  Quinoline and 8-hydroxyquinoline(8-HQ) are major industrial chemicals. Quinoline is also a constituent of automobile exhaust, tobacco smoke and indoor air pollution. Studies have shown that quinoline is a hepatocarcinogen and potent mitogen in rats and mice. 8-HQ, however, failed to induce hepatic carcinogenesis when tested in rodent chronic boassays, and is not known to produce a mitogenic response in rat liver. The following study was conducted to assess the comparative effects of quinoline and 8-HQ on hepatocyte function. Exposure to quinoline for 3 hrs depleted hepatocyte glutathione (GSH) levels in a dose-dependent manner. The addition of 500 uM quinoline depleted GSH levels to about 60 % of control values after 3 hrs. Quinoline did not induce the formation of oxidized glutathione (GSSG) at the concentrations tested. Exposure to 500uM 8-HQ for 3 hrs also depleted hepatocyte GSH to about 70% of control values, but GSH loss could be accounted for by increases in GSSG levels. Lower concentrations of 8-HQ did not deplete glutathione levels at the 3 hr timepoint. Both compounds induced increases in single strand DNA breaks at selected concentrations as judged by alkaline elution analysis, but 8-HQ induced increases in DNA single-strand breaks were associated with cytotoxic concentrations of 8HQ. Hepatocytes cultured for 48 hrs and subsequently exposed to quinoline or 8-HQ exhibited increased levels of ornithine decarboxylase activity 4 hrs after the addition of the chemicals. The result of this study suggests that quinoline and 8-hydroxyquinoline have significantly different effects on hepatocyte function. [Description provided by NIOSH]
• Subjects:
  Biological Assay Digestive System Digestive System Diseases DNA Environmental Exposure Environmental Monitoring Hazardous Substances Laboratories Liver Occupational Health Safety Toxicology

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• Keywords:
  Bioassays DNA-damage Exposure-assessment Exposure-levels Hepatic-microsomal-enzymes Industrial-emissions Industrial-exposures Laboratory-testing Liver-damage Liver-enzymes Liver-function Toxic-effects Toxins

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• ISSN:
  0731-9193
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DBBS - Division of Biomedical and Behavioral Sciences
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  14
• Issue:
  1
• NIOSHTIC Number:
  nn:20035547
• Citation:
  Toxicologist 1994 Mar; 14(1):193
• CAS Registry Number:
  Quinoline (CAS RN 91-22-5)
• Federal Fiscal Year:
  1994
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 33rd Annual Meeting, March 13-17, 1994, Dallas, Texas
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:155ab559adcf7062bed5d68fe3462c473a659100b7949ecd18109e9be88a6b9a60be86a143f8097eb66f4c62c57111b7fd94c305f8bf45ac43da224f6ba43c01
• Download URL:
  https://stacks.cdc.gov/view/cdc/187371/cdc_187371_DS1.pdf
• File Type:
  [PDF - 245.76 KB ]