Increased Gene Copy Number May Alter E2F1 Activity in Melanoma Growth

Details

• Personal Author:
  Beas A ; Goulet AC ; Jefferson AM ; Lowry DT ; Nelson MA ; Reynolds SH ; Sargent L ; Senft JR
• Description:
  The identification of recurring translocations and unique chromosome break points in melanoma will aid in the identification of the genes that are important in the neoplastic process. Previous studies by our group identified translocations der(12)t(12;20) in malignant melanoma cells. The transcription factor E2F1 maps to 20q11. Deregulated E2F transcriptional activity has been associated with the autonomous growth of melanoma cells, but the molecular basis has not yet been elucidated. To this end, we investigated E2F1 gene copy number and structure in nine different early passage human melanoma cell lines. Fluorescent in situ hybridization analysis using a specific E2F1 probe indicated increased E2F1 gene copies in several melanoma cell lines compared to normal melanocytes. The FISH observations were confirmed by comparative genomic hybridization array to BAC clones and Southern Blot analysis. In addition, Westem blot analysis demonstrated increased E2F1 and DP-1 protein levels in 8 out of 9 melanoma cells compared to normal melanocytes. These data suggests that the release of E2F activity by elevated E2F1 gene copy numbers may playa functional role in melanoma growth. [Description provided by NIOSH]
• Subjects:
  Carcinogens Genetics Neoplasms Occupational Health Safety Skin Skin Diseases
• Keywords:
  Cancer Carcinogenesis Cell-biology Cell-damage Cell-function Cellular-function Genetic-disorders Genetic-factors Skin-cancer Skin-diseases Skin-disorders Skin-tumors Tumorigenesis Tumors

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• ISSN:
  0197-016X
• Publisher:
  American Association for Cancer Research
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  44
• NIOSHTIC Number:
  nn:20035518
• Citation:
  Proceedings of the American Association for Cancer Research (AACR) 94th Annual Meeting, July 11-14, 2003, Washington, DC. 2nd edition. Philadelphia, PA: American Association for Cancer Research, 2003 Jul; 44:238
• Federal Fiscal Year:
  2003
• Peer Reviewed:
  False
• Source Full Name:
  Proceedings of the American Association for Cancer Research (AACR) 94th Annual Meeting, July 11-14, 2003, Washington, DC
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:e53488a6488d8b7b1706a46cbc721ca4e92fe4b2b2a8541c1070daf12f79712d6fe5b20f9b70b94ae658969656427fb2141f6135b48304335bb58eac43fd5121
• Download URL:
  https://stacks.cdc.gov/view/cdc/187350/cdc_187350_DS1.pdf
• File Type:
  [PDF - 251.10 KB ]