The Limits of Testing Particle-Mediated Oxidative Stress In Vitro in Predicting Diverse Pathologies; Relevance for Testing of Nanoparticles

Details

• Personal Author:
  Borm PJA ; Castranova, Vincent ; Donaldson K ; Gulumian M
• Description:
  In vitro studies with particles are a major staple of particle toxicology, generally used to investigate mechanisms and better understand the molecular events underlying cellular effects. However, there is ethical and financial pressure in nanotoxicology, the new sub-specialty of particle toxicology, to avoid using animals. Therefore an increasing amount of studies are being published using in vitro approaches and such studies require careful interpretation. We point out here that 3 different conventional pathogenic particle types, PM10, asbestos and quartz, which cause diverse pathological effects, have been reported to cause very similar oxidative stress effects in cells in culture. We discuss the likely explanation and implications of this apparent paradox, and its relevance for testing in nanotoxicology. [Description provided by NIOSH]
• Subjects:
  Aerosols Animals Dust Hazardous Substances Microbiology Occupational Health Particulate Matter Safety Toxicology
• Keywords:
  Aerosol-particles Biohazards Biological-effects Cell-function Cell-morphology Cellular-function Cellular-reactions Microscopic-analysis Molecular-biology Nanotechnology Oxidative-processes Particulate-dust Particulate-sampling-methods Particulates Respirable-dust Toxic-effects Toxic-materials Toxicopathology

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• ISSN:
  1743-8977
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  13
• Volume:
  6
• NIOSHTIC Number:
  nn:20035501
• Citation:
  Part Fibre Toxicol 2009 Apr; 6:13
• Contact Point Address:
  Ken Donaldson, MRC/University of Edinburgh Centre for Inflammation Research, ELEGI Colt Laboratory, Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK
• Email:
  ken.donaldson@ed.ac.uk
• Federal Fiscal Year:
  2009
• Peer Reviewed:
  True
• Source Full Name:
  Particle and Fibre Toxicology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:f6915428058e3ad55d2b0901e75ce9eaccc27ecf09b8e3c3ca4cfe9ac9929efd1ce134a5b1587095c958db76ec98f3bc59fbdcecc02bc00ba12da364e6312d2d
• Download URL:
  https://stacks.cdc.gov/view/cdc/187337/cdc_187337_DS1.pdf
• File Type:
  [PDF - 286.21 KB ]