Recognition of Live Phosphatidylserine-Labeled Tumor Cells by Dendritic Cells: A Novel Approach to Immunotherapy of Skin Cancer

Details

• Personal Author:
  Kagan VE ; Potapovich AI ; Shurin GV ; Shurin MR ; Tourkova IL ; Tyurina YY
• Description:
  Dendritic cells (DC) loaded with tumor antigens from apoptotic/necrotic tumor cells are commonly used as vaccines for cancer therapy. However, the use of dead tumor cells may cause both tolerance and immunity, making the effect of vaccination unpredictable. To deliver live tumor ''cargoes'' into DC, we developed a new approach based on the ''labeling'' of tumors with a phospholipid ''eat-me'' signal, phosphatidylserine. Expression of phosphatidylserine on live tumor cells mediated their recognition and endocytosis by DC resulting in the presentation of tumor antigens to antigen-specific T cells. In mice, topical application of phosphatidylserine-containing ointment over melanoma induced tumor-specific CTL, local and systemic antitumor immunity, and inhibited tumor growth. Thus, labeling of tumors with phosphatidylserine is a promising strategy for cancer immunotherapy. [Description provided by NIOSH]
• Subjects:
  Biochemistry Biological Factors Biomarkers Cytology Environmental Monitoring Hazardous Substances Immune System Microbiology Necrosis Neoplasms Occupational Health Safety Skin Skin Diseases

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• Keywords:
  Apoptosis Author Keywords: Phosphatidylserine Biochemical-indicators Biological-effects Biological-factors Biological-monitoring Cancer Cell-biology Cellular-reactions Dendritic Cells Endocytosis Exposure-assessment Exposure-levels Exposure-methods Immune-reaction Immunology Immunotoxins Laboratory-animals Lymphoma Melanoma Microscopic-analysis Microscopy Skin-cancer Statistical-analysis Topical Therapy Toxic-effects Tumor-inhibition Tumors

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• ISSN:
  0008-5472
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 3 ; OSHA Region 5 ; Pennsylvania ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  69
• Issue:
  6
• NIOSHTIC Number:
  nn:20035415
• Citation:
  Cancer Res 2009 Mar; 69(6):2487-2496
• Contact Point Address:
  Valerian E. Kagan or Michael R. Shurin, Center for Free Radical and Antioxidant Health, Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh, PA
• Email:
  kagan@pitt.edu
• Federal Fiscal Year:
  2009
• NORA Priority Area:
  Manufacturing
• Performing Organization:
  University of Pittsburgh at Pittsburgh
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Cancer Research
• End Date:
  20160630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:63e6736fc6eafc2a8c788dd02c466b89410c1802d4726f708b59ad5dc8fd67eb1be87aac0b9ba4f1ef65ad3610e3dc2a7f5b348c696ea52b8cc420dbe2f0ff1c
• Download URL:
  https://stacks.cdc.gov/view/cdc/187279/cdc_187279_DS1.pdf
• File Type:
  [PDF - 813.32 KB ]