Biodegradation of Single Walled Carbon Nanotubes Through Peroxidase Catalysis

Details

• Personal Author:
  Allen BL ; Kagan VE ; Kapralov AA ; Kisin ER ; Konduru NV ; Murray AR ; Shvedova AA ; Star A ; Vlasova II
• Description:
  Management of exposure to bioengineered single walled carbon nanotubes (SWCNT) is becoming a major environmental and health challenge. Because of unique characteristics, SWCNT have found increased applications in various fields of science and technology. With increased risk of human and environmental exposure, strategies to degrade SWCNT are also garnering interest. Here, we show - for the first time - that two peroxidases, namely myeloperoxidase and horseradish peroxidase are effective in catalyzing the biodegradation of SWCNT. A systematic characterization of resulting products of degradation was performed using transmission electron microscopy (TEM), dynamic light scattering (DLS), gel electrophoresis, mass spectrometry, UV-Vis-NIR spectroscopy and thermogravimetric analysis (TGA). We also demonstrate that unlike naïve non-biodegraded nanoparticles, the peroxidase degraded particles did not elicit pulmonary inflammatory response in mice as evidenced by the release of pro-inflammatory cytokines and the content of neutrophils (PMN) in bronchoalveolar lavage. These results mark a novel approach to employ peroxidase catalysis for directed biodegradation of carbon nanotubes in biofluids/tissues as well as in environmental settings. [Description provided by NIOSH]
• Subjects:
  Biological Factors Cytology Environmental Exposure Environmental Health Environmental Monitoring Environmental Pollution Hazardous Substances Irritants Laboratories Lung Lung Diseases Microbiology Occupational Health Particulate Matter Respiratory System Respiratory Tract Diseases Risk Assessment Risk Factors Safety

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• Keywords:
  Biological-effects Biological-factors Cell-biology Cellular-reactions Environmental-exposure Environmental-hazards Environmental-health Exposure-assessment Exposure-levels Exposure-methods Health-hazards Inhalation-studies Laboratory-animals Lung-cells Lung-disorders Lung-irritants Microscopic-analysis Microscopy Nanotechnology Oxidative-processes Particle-aerodynamics Particulate-dust Particulates Respiratory-irritants Respiratory-system-disorders Risk-factors

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• ISSN:
  1096-6080
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; Pennsylvania ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  108
• Issue:
  1
• NIOSHTIC Number:
  nn:20035307
• Citation:
  Toxicologist 2009 Mar; 108(1):458
• CAS Registry Number:
  Carbon (CAS RN 7440-44-0)
• Federal Fiscal Year:
  2009
• NORA Priority Area:
  Manufacturing
• Performing Organization:
  University of Pittsburgh at Pittsburgh
• Peer Reviewed:
  False
• Start Date:
  20050701
• Source Full Name:
  The Toxicologist. Society of Toxicology 48th Annual Meeting and ToxExpo, March 15-19, 2009, Baltimore, Maryland
• End Date:
  20160630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:e9f75c7069e5a1d2cad8e8a69c85481ed63d6d3e2a31136cf3ff1f18f9cc702420cc7d885052f9434580a7b442f214ce29e56a54b328e3d49f58748fc10803d3
• Download URL:
  https://stacks.cdc.gov/view/cdc/187215/cdc_187215_DS1.pdf
• File Type:
  [PDF - 1.85 MB ]