Nanoparticle Dispersion Method Natural Lung Surfactant

Details

• Personal Author:
  Castranova, Vincent ; Hall J ; Li M ; Li S ; Li T ; Mercer RR ; Rojanasakul Y ; Wang L ; Wu N
• Description:
  Nanomaterials, as a class of small-scale (<100 nm) substances with unique mechanical, optical and electrical properties, are increasingly being used in a wide range of industries. Their unique properties present new challenges to understanding the toxicity of these materials to humans and the environment. Lung is the major target organ for airborne nanoparticles. In vitro and in vivo exposure studies often rely on the use of suspended nanoparticle preparations. However, nanoparticles suspended in culture medium or physiologic saline solution tend to form micrometersized aggregates. Increasing evidence indicates that the degree of dispersion of nanoparticles has a strong influence on their biological activities. In this study, we test a new method of nanoparticle dispersion using natural lung surfactant, Survanta, as a dispersing agent. Dose dependence studies of Survanta were performed on single-walled carbon nanotube (SWCNT) dispersion. Our results show that Survanta at a concentration of 150 microg/ml, which is comparable to that found in normal rodent lungs, was optimal in dispersing SWCNT (0.1 mg/ml), producing well dispersed preparations as analyzed by microscopic and light scattering methods. This dose of Survanta was found to be non-toxic and non-inflammatory in vivo and in vitro when used alone, and did not mask the bioactivity of SWCNT. We also found that the dispersed form of SWCNT was more effective in inducing cytotoxicity and lung fibrosis than the non-dispersed form, indicating the importance of nanoparticle dispersion on biological activities. Since Survanta is commercially available and its one step nanoparticle dispersion is simple and rapid, this method provides major advantages over existing methods of nanoparticle dispersion. Furthermore, our stability studies showed that Survanta-dispersed nanoparticles remain well dispersed for months and upon dilution with aqueous medium. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Biological Factors Cytology Environmental Monitoring Irritants Laboratories Lung Lung Diseases Microbiology Occupational Health Particulate Matter Respiratory Hypersensitivity Respiratory System Respiratory Tract Diseases Safety

  More +
• Keywords:
  Airborne-particles Biological-effects Biological-factors Cell-alteration Cell-biology Cell-morphology Cellular-reactions Exposure-assessment Exposure-levels Exposure-methods Inhalation-studies Laboratory-animals Lung-cells Lung-irritants Microscopic-analysis Nanotechnology Particle-aerodynamics Particulates Pulmonary-disorders Pulmonary-function Pulmonary-system-disorders Respiratory-hypersensitivity Respiratory-irritants Statistical-analysis

  More +
• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  108
• Issue:
  1
• NIOSHTIC Number:
  nn:20035306
• Citation:
  Toxicologist 2009 Mar; 108(1):458
• Federal Fiscal Year:
  2009
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 48th Annual Meeting and ToxExpo, March 15-19, 2009, Baltimore, Maryland
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c5245d08f7d53d8c37f61be8d184206dc08f0e987fb28280b9322e953a4b8c1a5cf502773019a270622212345ff919e24a65bc38d25c97c15ca0c969a8a8af44
• Download URL:
  https://stacks.cdc.gov/view/cdc/187214/cdc_187214_DS1.pdf
• File Type:
  [PDF - 1.85 MB ]