Calcium-Dependent Vasodilation Is Impaired in Coronary Arterioles After Nanoparticle Inhalation

Details

• Personal Author:
  Castranova, Vincent ; Chen B ; Cumpston J ; Frazer D ; LeBlanc AJ ; Nurkiewicz T
• Description:
  Epidemiological studies have shown that exposure to particle pollution is associated with an increased risk for myocardial infarction (MI). This laboratory has shown in skeletal muscle that nanoparticle exposure produces significantly greater microvascular dysfunction than larger particles of the same composition. However, it remains unclear if coronary microvascular endothelial function is affected to a similar degree. Rats were exposed to filtered air (control) or TiO2 nanoparticles (primary particle diameter, approximately 21 nm) via inhalation at concentrations relevant to ambient air pollution (9.5 mu g measured pulmonary deposition). Coronary arterioles (approximately 150 mu m in diameter) were isolated from the left anterior descending artery distribution and responses to flow (FID) (5-25 uL/min), acetylcholine (ACh, 10(-7) - 10(-5) M), and the Ca(2+) ionophore, A23187 (10(-8) - 10(-6) M), were assessed. Endothelium-dependent FID was preserved in coronary arterioles from rats exposed to nano-TiO2 compared to control rats. Conversely, a profound vasoconstriction (16+/-14, % constriction) resulted from cumulative additions of ACh in arterioles from rats exposed to nanoparticles, whereas control rats responded by vasodilation (73+/-4, % dilation). Similarly, nanoparticle exposure impaired arteriolar dilation to A23187 as compared to control rats. Sodium nitroprusside (10(-3) M) produced comparable arteriolar dilation in both groups, indicating that vascular smooth muscle NO responsiveness remains intact after nanoparticle exposure. These results suggest that nanoparticle exposure significantly impairs Ca(2+)-dependent microvascular responses to ACh and A23187, whereas responsiveness to shear stress is preserved. It is probable that such disturbances in coronary microvascular function contribute to the cardiac events associated with particle pollution exposure. [Description provided by NIOSH]
• Subjects:
  Biological Factors Cardiovascular Diseases Cardiovascular System Environmental Monitoring Epidemiology Laboratories Microbiology Musculoskeletal Diseases Musculoskeletal System Occupational Health Particulate Matter Respiratory System Safety

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• Keywords:
  Biological-effects Biological-factors Cardiovascular-function Cardiovascular-system Cardiovascular-system-disorders Exposure-assessment Exposure-levels Exposure-methods Inhalation-studies Laboratory-animals Microscopic-analysis Musculoskeletal-system Musculoskeletal-system-disorders Nanotechnology Particle-aerodynamics Particulate-dust Particulates Pollutants Pollution Skeletal-disorders Skeletal-system-disorders Statistical-analysis

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  278-279
• Volume:
  108
• Issue:
  1
• NIOSHTIC Number:
  nn:20035258
• Citation:
  Toxicologist 2009 Mar; 108(1):278-279
• Federal Fiscal Year:
  2009
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 48th Annual Meeting and ToxExpo, March 15-19, 2009, Baltimore, Maryland
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:8978afaf797cc731e1bbb7a3c3af96f75073f600203e4e4977ec300497343eacd1d7dccb20fe235cb060f804da1a575634286d1e120829b0718887416669f5d1
• Download URL:
  https://stacks.cdc.gov/view/cdc/187173/cdc_187173_DS1.pdf
• File Type:
  [PDF - 1.59 MB ]