Real-Time Measurement of Fluorescence Spectra from Single Airborne Biological Particles

Details

• Personal Author:
  Bottiger J ; Chang RK ; Chen BT ; Feather G ; Hill SC ; Holler S ; Niles S ; Orr CS ; Pan YL ; Pinnick RG
• Description:
  Improved real-time methods for characterizing airborne biological particles are needed. Here we review our efforts in developing techniques for measuring the laser-induced fluorescence (total and spectrally dispersed) of individual airborne particles, and describe our present system, which can measure fluorescence spectra of single micrometer-sized bioaerosol particles with good signal-to-noise ratios. We demonstrate the capability of this system by showing measured spectra of a variety of airborne particles generated in the laboratory from road dust, ammonium sulfate, Bacillus subtilis and other bacteria prepared under various conditions, allergens, cigarette smoke, and chicken-house dust. These spectra illustrate the capability of the system to distinguish between some biological and nonbiological aerosols, and among several types of laboratory-generated biological aerosols. We suggest improvements needed to make our system field portable. [Description provided by NIOSH]
• Subjects:
  Aerosols Air Pollutants, Occupational Biological Products Occupational Health Particulate Matter Safety
• Keywords:
  Aerosol-particles Aerosol Detection Airborne-particles Analytical-instruments Analytical-processes Author Keywords: Bioaerosols Biological-material Biological Aerosols Fluorescence Spectroscopy Instrumentation Single-particle Fluorescence

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• ISSN:
  1086-900X
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  221-239
• Volume:
  3
• Issue:
  4
• NIOSHTIC Number:
  nn:20035231
• Citation:
  Field Anal Chem Tech 1999 Apr; 3(4-5):221-239
• Federal Fiscal Year:
  1999
• Peer Reviewed:
  True
• Source Full Name:
  Field Analytical Chemistry and Technology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:3cba150a97addcb7fc5e8516e7eeb93328ecbf88221ba11e25afd90f121a8fee0cb1462a6d177f9d78bf5e18c47c5fe3c96db03540177a00e0cfb171f070625a
• Download URL:
  https://stacks.cdc.gov/view/cdc/187151/cdc_187151_DS1.pdf
• File Type:
  [PDF - 312.37 KB ]