Increased Cell Proliferation in Spleen and Lymph Nodes Peripheral to Contact Allergen Application Site

Details

• Personal Author:
  Anderson, Stacey E. ; Beezhold, Donald H. ; Butterworth LF ; Chipinda I ; Siegel, Paul D. ; Anderson, Stacey E. ; Beezhold, Donald H. ; Butterworth LF ; Chipinda I ; Siegel, Paul D.
• Description:
  The local lymph node assay (LLNA) is widely used to identify chemicals that are contact sensitizers. The assay involves dosing mice with the chemical on both ears and pooling the superficial parotid lymph nodes for assessment of lymphocyte proliferation as a marker of sensitization. The present study explored potential reduction in animal usage by dosing one ear with the allergen and the other with vehicle-only. The respective draining lymph nodes were processed separately for tritiated thymidine (3H-TdR) incorporation. Cell proliferation in proper axillary and renal nodes, as well as in the spleen was also assessed. Cross-contamination of the chemicals from the dosed ears to other parts of the body via preening was prevented by dosing restrained animals and washing off the residual chemical with saline after 4 h. Dosing the left ear with 0.02% oxazolone (OX) on unrestrained animals resulted in marked cell proliferation in its draining lymph node (stimulation index, SI = 12.8) and in the lymph node draining the contra-lateral vehicle-dosed ear (SI = 6), as well as the proper axillary lymph nodes (SI = 3.3). Increased 3H-TdR incorporation was not observed in the renal lymph nodes (SI = 1.1). Similar stimulation of cells was observed in the lymph node draining the ear contra-lateral to the 30% hexylcinnamaldehyde (HCA)-dosed ear. Increased proliferative activity was observed in contra-lateral draining lymph nodes of restrained mice demonstrating that these results cannot be attributed to cross-contamination of adjacent skin. A significant increase in proliferation of splenocytes was also observed. It is concluded that dermal application of a contact allergen, as exemplified by OX and HCA, may induce cell proliferation in the neighboring lymph nodes and spleen indicative of hapten and/or haptenated proteins diffusing through the skin to peripheral nodes and the blood to produce systemic sensitization. It is also possible that lymphatic capillaries may communicate between the left and right side of the mouse head. Thus the contra-lateral draining superficial parotid node cannot be used as a control for application of contact allergen to a single ear in a modified LLNA. [Description provided by NIOSH]
• Subjects:
  Animals Biological Factors Biomarkers Blood Chemistry Ear Hematologic Tests Hypersensitivity Laboratories Occupational Health Risk Assessment Safety Sense Organs Skin Skin Absorption

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• Keywords:
  Animal-studies Author Keywords: Contact Sensitizer Biological-effects Biological-factors Biological-monitoring Blood-analysis Blood-cells Blood-sampling Blood-stream Cell-biology Cell-damage Cell-function Cell-growth Cell-metabolism Cellular-function Cellular-reactions Chemical-analysis Chemical-composition Chemical-hypersensitivity Chemical-reactions Dose-response Ear-disorders Ears Laboratory-animals Laboratory-testing Local Lymph Node Assay Lymph-nodes Lymphatic-system Peripheral Lymph Nodes Physical-reactions Quantitative-analysis Risk-analysis Sensitization Skin-absorption Skin-exposure Skin-sensitivity Spleen Spleen-disorders Splenic-tissue

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• ISSN:
  0300-483X
• Document Type:
  Text
• Genre:
  Journal Article ; Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH) ; National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health ; Workplace Safety & Health
• Location:
  North America ; North America
• Pages in Document:
  113-116
• Volume:
  257
• Issue:
  3
• NIOSHTIC Number:
  nn:20035118
• Citation:
  Toxicology 2009 Mar; 257(3):113-116
• Contact Point Address:
  Itai Chipinda, Health Effects Laboratory Division, Allergy and Clinical Immunology Branch, NIOSH, 1095 Willowdale Road, Morgantown, WV 26505
• Email:
  IChipinda@cdc.gov
• Federal Fiscal Year:
  2009
• NORA Priority Area:
  Services ; Services
• Peer Reviewed:
  True
• Source Full Name:
  Toxicology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:8747644c61ae2b9bcc7626e36f8c52bda167c310befd1eca5e62b06f906419effb1a44d45846c6515fd697d13ab364841332f7ed4c18392ccae9102a55cccacb
• Download URL:
  https://stacks.cdc.gov/view/cdc/187076/cdc_187076_DS1.pdf
• File Type:
  [PDF - 179.82 KB ]