Simultaneous Measurement of Mechanical Responses and Transepithelial Potential Difference and Resistance, in Guinea-Pig Isolated, Perfused Trachea Using a Novel Apparatus: Pharmacological Characterization

Details

• Personal Author:
  Dowdy JA ; Fedan, Jeffery S. ; Jing Y ; Van Scott MR
• Description:
  The isolated, perfused trachea preparation has been used to compare reactivity of the intact airway in response to differential exposure of the mucosal (intraluminal) and serosal (extraluminal) surfaces to contractile and relaxant agonists and other agents, and to gain insight into the modulatory role of the epithelium and the pathways involved. The apparatus has also been configured for simultaneous measurement of transepithelial potential difference and changes in tracheal diameter, thereby providing parallel observations of epithelial and smooth muscle function and reactivity to drugs. The transepithelial potential difference is a product of transepithelial resistance and short circuit current, and the present study describes a novel isolated, perfused tracheal apparatus which allows simultaneous measurement of transepithelial potential difference, transepithelial resistance and mechanical responses of the smooth muscle. The apparatus was validated using well-known ion transport inhibitors [intraluminal amiloride and 5-nitro-2-(3-phenylpropyl-amino) benzoic acid (NPPB), extraluminal ouabain and bumetanide], bronchoactive agonists (extraluminal methacholine, histamine and terbutaline), and osmolytes (intraluminal D-mannitol and NaCl) to induce epithelium-derived relaxing factor-mediated relaxations. This apparatus will facilitate investigation of interactions between the epithelium and smooth muscle in airways that retain their in situ structure, and signaling mechanisms potentially involved in the regulation of airway smooth muscle tone. [Description provided by NIOSH]
• Subjects:
  Musculoskeletal System Occupational Health Safety
• Keywords:
  Airway-obstruction Cell-function Cellular-function Mucous-membranes Muscle-function Muscle-physiology Muscle-relaxants Muscle-tissue Reaction-rates
• ISSN:
  0014-2999
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  98-103
• Volume:
  598
• Issue:
  1
• NIOSHTIC Number:
  nn:20034726
• Citation:
  Eur J Pharmacol 2008 Nov; 598(1-3):98-103
• Contact Point Address:
  Jeffrey S. Fedan, Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505
• Email:
  jsf2@cdc.gov
• Federal Fiscal Year:
  2009
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  True
• Source Full Name:
  European Journal of Pharmacology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:eb442cbd7b128d504354df87645c79649fa43f9dc6ccd944902f42662aa08de460351e7cfa1b22b7d1551516cd81c64e6a15a084bf1c12b6f4623e930ee30b06
• Download URL:
  https://stacks.cdc.gov/view/cdc/186828/cdc_186828_DS1.pdf
• File Type:
  [PDF - 979.75 KB ]